ROR1 Double Nickase Plasmid (h): sc-401841-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ROR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ROR1 Double Nickase Plasmid (h) and ROR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ROR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ROR1 Antibody (60-D): sc-130386
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ROR1 Double Nickase Plasmid (h) | sc-401841-NIC | 20 µg | $410.00 | |||
ROR1 Double Nickase Plasmid (h2) | sc-401841-NIC-2 | 20 µg | $410.00 |
ROR1 (receptor tyrosine kinase-like orphan receptor 1) encodes a single-pass transmembrane receptor in the ROR family that participates in non-canonical Wnt signaling, including ligand-dependent responses to WNT5A. ROR1 modulates cellular polarity, migration, cytoskeletal remodeling, and survival programs through pathway cross-talk involving Rho-family GTPases, PI3K/AKT, MAPK, and NF-κB-associated signaling nodes. Although expression is tightly regulated during development, aberrant ROR1 expression and signaling have been associated with altered differentiation states and invasive phenotypes in multiple disease contexts. These properties make ROR1 a useful target for interrogating Wnt pathway wiring, receptor-proximal signaling, and mechanisms governing motility and cell-state plasticity.
ROR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ROR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ROR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ROR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ROR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.