RNF6 Antibody (3B1): sc-517144
- RNF6 Antibody (3B1) is a mouse monoclonal IgG2a κ RNF6 antibody provided at 100 µg/ml
- raised against amino acids 1-100 representing partial length RNF6 of human origin
- recommended for detection of RNF6 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RNF6 Antibody (3B1). This work is in progress.
RNF6 Antibody (3B1) is a mouse monoclonal IgG2a kappa light chain antibody that detects RNF6 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNF6 Antibody (3B1) is available as a non-conjugated anti-RNF6 antibody. RNF6, also known as ring finger protein (C3H2C3 type) 6, is a 685 amino acid protein characterized by a RING-type zinc finger motif, which is crucial for function in the ubiquitination pathway of protein degradation. This motif consists of a conserved cysteine-rich domain capable of binding two zinc atoms, a feature essential for structural integrity and functional activity. RNF6 plays a significant role in regulating local growth cone actin dynamics and cellular concentration of LIMK-1, which is vital for various cellular processes, including cell migration and proliferation. RNF6 has been implicated in tumor suppression, as mutations and deletions have been identified in esophageal squamous cell carcinoma, highlighting potential as a biomarker for cancer research. The gene encoding RNF6 is located on human chromosome 13q12.13 and produces a splice variant highly expressed in adult testis, indicating relevance in reproductive biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RNF6 Antibody (3B1) References:
- Cloning and characterization of RNF6, a novel RING finger gene mapping to 13q12. | Macdonald, DH., et al. 1999. Genomics. 58: 94-7. PMID: 10331950
- RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. | Lorick, KL., et al. 1999. Proc Natl Acad Sci U S A. 96: 11364-9. PMID: 10500182
- Gene control in germinal differentiation: RNF6, a transcription regulatory protein in the mouse sertoli cell. | Lopez, P., et al. 2002. Mol Cell Biol. 22: 3488-96. PMID: 11971979
- Identification of somatic mutations of the RNF6 gene in human esophageal squamous cell carcinoma. | Lo, HS., et al. 2002. Cancer Res. 62: 4191-3. PMID: 12154016
- The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones. | Tursun, B., et al. 2005. Genes Dev. 19: 2307-19. PMID: 16204183
- Cloning and identification of a novel RNF6 transcriptional splice variant Spg2 in human development. | Zhu, L., et al. 2008. Sci China C Life Sci. 51: 302-7. PMID: 18368307
- The RING finger domain: a recent example of a sequence-structure family. | Borden, KL. and Freemont, PS. 1996. Curr Opin Struct Biol. 6: 395-401. PMID: 8804826
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF6 Antibody (3B1) | sc-517144 | 100 µg/ml | $316.00 |