RNF181 Antibody (F-9E): sc-101120
- RNF181 Antibody (F-9E) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 100 µg/ml
- raised against recombinant RNF181 of human origin
- recommended for detection of RNF181 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RNF181 Antibody (F-9E). This work is in progress.
RNF181 Antibody (F-9E) is a mouse monoclonal IgG2a antibody that detects RNF181 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). RNF181, a 153 amino acid protein, features a RING-type zinc finger motif, which is crucial for its role in the ubiquitination pathway of protein degradation. This motif consists of a conserved cysteine-rich domain capable of binding two zinc atoms, allowing RNF181 to participate in the regulation of protein turnover and cellular homeostasis. The importance of RNF181 extends to its involvement in the cellular response to DNA damage, where RNF181 undergoes auto-ubiquitination and is phosphorylated by ATM or ATR kinases, highlighting its role in maintaining genomic integrity. RNF181 exhibits the highest expression levels in the liver and heart, with moderate expression in the placenta, lung, kidney, and pancreas, and lower levels in the brain and skeletal muscle. The conservation of the RNF181 gene across various species, including chimpanzees, dogs, cows, mice, rats, zebrafish, and fruit flies, underscores its fundamental biological significance, as RNF181 maps to human chromosome 2p11.2. Anti-RNF181 antibody (F-9E) is an essential tool for researchers investigating the mechanisms of protein degradation and the cellular response to stress.
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RNF181 Antibody (F-9E) References:
- Protein microarray characterization of the S-nitrosoproteome. | Lee, YI., et al. 2014. Mol Cell Proteomics. 13: 63-72. PMID: 24105792
- Interstitial microduplication at 2p11.2 in a patient with syndromic intellectual disability: 30-year follow-up. | Jun, KR., et al. 2014. Mol Cytogenet. 7: 52. PMID: 25295072
- Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. | Pedersen, SM., et al. 2015. Mol Cell Biol. 36: 794-808. PMID: 26711259
- Correction for Pedersen et al., Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181. | Pedersen, SM., et al. 2016. Mol Cell Biol. 36: 1322. PMID: 27034408
- Insights into the ubiquitin-proteasome system of human embryonic stem cells. | Saez, I., et al. 2018. Sci Rep. 8: 4092. PMID: 29511261
- RN181 is a tumour suppressor in gastric cancer by regulation of the ERK/MAPK-cyclin D1/CDK4 pathway. | Wang, S., et al. 2019. J Pathol. 248: 204-216. PMID: 30714150
- RNF181 modulates Hippo signaling and triple negative breast cancer progression. | Zhou, R., et al. 2020. Cancer Cell Int. 20: 291. PMID: 32655323
- The ubiquitin ligase RNF181 stabilizes ERα and modulates breast cancer progression. | Zhu, J., et al. 2020. Oncogene. 39: 6776-6788. PMID: 32973333
- Synergistic effects of α-Mangostin and sorafenib in hepatocellular carcinoma: New insights into α-mangostin cytotoxicity. | Wang, TT., et al. 2021. Biochem Biophys Res Commun. 558: 14-21. PMID: 33894673
- Weighted Co-Expression Network Analysis Identifies RNF181 as a Causal Gene of Coronary Artery Disease. | Dang, R., et al. 2021. Front Genet. 12: 818813. PMID: 35222523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF181 Antibody (F-9E) | sc-101120 | 100 µg/ml | $339.00 |