RNF170 Double Nickase Plasmid (h): sc-410169-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF170 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF170 Double Nickase Plasmid (h) and RNF170 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF170. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF170 Double Nickase Plasmid (h) | sc-410169-NIC | 20 µg | $410.00 |
RNF170 encodes an endoplasmic reticulum–resident RING finger E3 ubiquitin ligase that supports protein quality control by promoting ubiquitination and proteasomal turnover of select membrane-associated substrates. Through ER-associated degradation (ERAD) and ubiquitin-dependent signaling, RNF170 contributes to proteostasis and can influence calcium and stress-responsive pathways linked to ER function. Altered RNF170 activity has been associated with dysregulated ubiquitin signaling and has been studied in the context of neurodegeneration and other disorders where ER homeostasis is perturbed. As a result, RNF170 is a useful target for probing ubiquitin ligase specificity, ER stress responses, and downstream signaling networks in human cells.
RNF170 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF170 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF170. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF170 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF170-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.