RNF157 Double Nickase Plasmid (h): sc-407283-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF157 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF157 Double Nickase Plasmid (h) and RNF157 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF157. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF157 Double Nickase Plasmid (h) | sc-407283-NIC | 20 µg | $410.00 | |||
RNF157 Double Nickase Plasmid (h2) | sc-407283-NIC-2 | 20 µg | $410.00 |
RNF157 encodes a RING finger E3 ubiquitin ligase that contributes to ubiquitin-dependent protein turnover and signaling control in human cells. Through its catalytic role in ubiquitination, RNF157 can influence proteostasis, stress responses, and cell fate decisions by modulating the stability and activity of downstream substrates. E3 ligases such as RNF157 intersect with pathways governing neuronal maintenance and apoptosis-related processes, making RNF157 of interest in studies of neurobiology and cellular survival mechanisms. Altered ubiquitin pathway regulation, including dysregulation of E3 ligases, is frequently explored in the context of cancer-associated signaling, neurodegenerative mechanisms, and other conditions linked to impaired protein homeostasis.
RNF157 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF157 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF157. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF157 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF157-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.