RNF150 Double Nickase Plasmid (h): sc-412810-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF150 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF150 Double Nickase Plasmid (h) and RNF150 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF150. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF150 Double Nickase Plasmid (h) | sc-412810-NIC | 20 µg | $410.00 |
RNF150 encodes RING finger protein 150, a putative E3 ubiquitin ligase that contributes to ubiquitin-dependent regulation of protein stability and signaling. Through modulation of substrate ubiquitination, RNF150 is positioned to influence proteostasis, turnover of regulatory proteins, and crosstalk with pathways such as endosomal trafficking and stress-responsive signaling cascades. Altered ubiquitin pathway activity is frequently linked to dysregulated cell-cycle control, inflammatory signaling, and genome maintenance, making RNF150 of interest for mechanistic studies in these contexts. Expression and genomic variation in RNF150 have been explored in datasets spanning multiple disease areas, supporting its utility as a candidate gene for functional annotation and pathway mapping in human cells.
RNF150 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF150 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF150. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF150 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF150-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.