RIPA Lysis Buffer System (w/out PMSF) A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System (w/out PMSF)

RIPA Lysis Buffer System (w/out PMSF) is rated 5.0 out of 5 by 1.
  • y_2020, m_10, d_29, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_364162, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 139ms
  • REVIEWS, PRODUCT
5
1
4
0
3
0
2
0
1
0
Alternate Names: RIPA Lysis Buffer System (w/out PMSF) also known as PMSF Protease Inhibitor
Application: RIPA Lysis Buffer System (w/out PMSF) is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

RIPA (Radioimmunoprecipitation) Lysis Buffer (w/out PMSF) is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

RIPA Lysis Buffer System (w/out PMSF) is a high quality product that includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-364162, 50 mL - Components supplied in three vials:
VIAL 1: 50 mL 1X lysis buffer
VIAL 2: 500 μL protease inhibitor cocktail in DMSO
VIAL 3: 500 μL (100mM) sodium orthovanadate in water

sc-364162A, 500 mL - Components supplied in three vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml protease inhibitor cocktail in DMSO
VIAL 3: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F. et al. 1982. Int. J. Cancer. 29(1): 69-76. PMID: 6277805 2. DeSeau, V. et al. 1987. J. Cell. Biochem. 35(2): 113-128. PMID: 2448318 3. Bolen, J.B. et al. 1987. Oncogene Res. 1(2): 149-168. PMID: 2453014 4. Ngoka, L.C. 2008. Proteome Sci. 6

Usage :
• Immediately prior to lysing cells, combine 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

• Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

RIPA Lysis Buffer System (w/out PMSF)  Product Citations

See how others have used RIPA Lysis Buffer System (w/out PMSF). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 23 total

PMID: # 31068616  Myszor, IT.|Parveen, Z.|Ottosson, H.|Bergman, P.|Agerberth, B.|Strömberg, R.|Gudmundsson, GH.| et al. 2019. Sci Rep. 9: 7114.

PMID: # 29868502  Cruz, R. et al. 2018. Front Cell Infect Microbiol. 8: 146.

PMID: # 26810190  Tong, WW. et al. 2016. Tumour Biol.

PMID: # 26342052  Shao, Y. et al. 2015. J Toxicol Sci. 40: 817-28.

PMID: # 25646597  Zhao, C. et al. 2015. International journal of oncology. 46: 1643-50.

PMID: # 25041469  Shi, SH. et al. 2015. Am. J. Reprod. Immunol. 73: 301-12.

PMID: # 25821818  Pająk, B. et al. 2015. BioMed research international. 2015: 746092.

PMID: # 25669938  Zhang, L. et al. 2014. Nature genetics. 46: 726-30.

PMID: # 24199849  Duan, X. et al. 2014. Clin Radiol. 69: 186-93.

PMID: # 23867821  Dufour, M. et al. 2013. Biochem. Biophys. Res. Commun. 438: 32-7.

Citations 1 to 10 of 23 total

Shoudl there be a protocol for sample prep for the procduct sc-364162? In my case there isn't any to find but we would want to know what the manufacturer reommends for incubation and centrifugation step. Can you please clarify? Thanks, Christine

Asked by: C Bosch
Thank you for your question. We recommend to combine 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer immediately before lysing cells to prepare complete RIPA. 3 ml complete RIPA per gram of tissue OR 1 ml complete RIPA per 2.0x107 cells in suspension OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate should be used. Gently agitate the solution for 15 minutes at 4° C before transferring the resulting lysate to a microcentrifuge tube.
Answered by: Tech Support Europe
Date published: 2019-07-30

Buongiorno, vorrei sapere a che temperatura conservare RIPAbuffer. Il prodotto è arrivato in ghiaccio secco e va conservato a -20°C. Dopo il primo scongelamento posso conservalo a 4°C? Grazie

Asked by: STEFANIA1
Thank you for your question. The buffer can be kept at 4 °C for short-term storage and should be frozen at -20 °C for long-term storage to ensure its viability. Grazie per la sua domanda. Il Tampone puó essere conservato a 4 °C solo per tempi brevi, mentre dovrebbe essere conservato a -20 °C per mantenere la stabilitá a lungo periodo.
Answered by: Technical Support Europe
Date published: 2019-01-23
  • y_2020, m_10, d_30, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_364162, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 133ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Various publications cite the use of RIPA Various publications cite the use of RIPA Lysis Buffer System to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. -SCBT Publication Review
Date published: 2015-01-07
  • y_2020, m_10, d_29, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_364162, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.