RIN3 CRISPR/Cas9 KO Plasmid (m): sc-432255
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RIN3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RIN3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIN3 CRISPR/Cas9 KO Plasmid (m) | sc-432255 | 20 µg | $397.00 |
Overview
Rin3 encodes RIN3, a Ras and Rab interactor that functions as a guanine nucleotide exchange factor for Rab5 family GTPases, linking receptor tyrosine kinase signaling to endocytic trafficking. In mouse cells, RIN3 participates in clathrin-mediated endocytosis, early endosome maturation, and regulation of receptor internalization and recycling, processes that shape downstream signaling amplitude and duration. Through these roles, Rin3 influences vesicle dynamics, membrane turnover, and cytoskeletal coordination during cell activation states. Altered RIN3 activity has been connected to disease-relevant mechanisms involving dysregulated endosomal trafficking and receptor signaling, supporting its study in pathways that contribute to inflammatory and neurodegenerative phenotypes.
RIN3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rin3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rin3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rin3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RIN3 protein expression.
This CRISPR knockout system enables efficient generation of Rin3-deficient cell models for investigation of RIN3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rin3 exon(s) critical for RIN3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rin3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RIN3 CRISPR/Cas9 KO Plasmid (m) and RIN3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rin3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RIN3 HDR Plasmid (m) and RIN3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rin3 homology arms to support homology-directed repair at defined Rin3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.