Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rictor Double Nickase Plasmid (h): sc-400710-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rictor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rictor Double Nickase Plasmid (h) and Rictor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RICTOR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rictor Antibody (H-11): sc-271081
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rictor Double Nickase Plasmid (h) | sc-400710-NIC | 20 µg | $410.00 | |||
Rictor Double Nickase Plasmid (h2) | sc-400710-NIC-2 | 20 µg | $410.00 |
RICTOR encodes rictor, an essential scaffolding component of mTOR complex 2 (mTORC2) that coordinates growth factor signaling with cellular metabolism, survival, and cytoskeletal organization. Through mTORC2-dependent phosphorylation of AGC kinases such as AKT (Ser473), SGK1, and PKC isoforms, rictor regulates insulin/PI3K signaling output, ion transport programs, and actin dynamics that influence migration and cell polarity. Dysregulated RICTOR/mTORC2 activity is frequently studied in contexts of oncogenic signaling, metabolic dysfunction, and stress-adaptive remodeling, where altered AKT pathway tuning can reshape proliferation and resistance phenotypes. As a result, RICTOR is widely used as a genetic entry point to dissect mTOR network topology and pathway crosstalk in human cell models.
Rictor Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RICTOR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RICTOR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RICTOR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RICTOR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.