Ribosomal Protein L8 Double Nickase Plasmid (m): sc-424161-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein L8 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ribosomal Protein L8 Double Nickase Plasmid (m) and Ribosomal Protein L8 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Rpl8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein L8 Double Nickase Plasmid (m) | sc-424161-NIC | 20 µg | $410.00 | |||
Ribosomal Protein L8 Double Nickase Plasmid (m2) | sc-424161-NIC-2 | 20 µg | $410.00 |
Mouse Rpl8 encodes ribosomal protein L8, a core component of the 60S large ribosomal subunit that supports ribosome biogenesis and efficient translation elongation. As part of the ribosomal machinery, RPL8 contributes to proteostasis programs that integrate with mTOR-regulated translation control and the cellular response to nutrient availability and stress. Perturbations in ribosomal proteins can disrupt global protein synthesis and trigger nucleolar stress pathways that intersect with cell-cycle regulation and p53 signaling. Because ribosome output influences proliferation, differentiation, and stress adaptation, Rpl8 is frequently studied in contexts where translational capacity and ribosome integrity shape disease-relevant phenotypes.
Ribosomal Protein L8 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Rpl8 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rpl8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rpl8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rpl8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.