Ribosomal Protein L10a Double Nickase Plasmid (h): sc-417441-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein L10a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ribosomal Protein L10a Double Nickase Plasmid (h) and Ribosomal Protein L10a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RPL10A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein L10a Double Nickase Plasmid (h) | sc-417441-NIC | 20 µg | $410.00 | |||
Ribosomal Protein L10a Double Nickase Plasmid (h2) | sc-417441-NIC-2 | 20 µg | $410.00 |
RPL10A encodes ribosomal protein L10a, a core component of the 60S large ribosomal subunit that supports ribosome biogenesis and peptide elongation during cytosolic translation. By contributing to assembly and function of the translational machinery, RPL10A influences proteostasis and cellular growth programs that intersect with stress-response signaling and cell-cycle control. Altered ribosomal protein dosage or function can perturb global and transcript-selective translation, linking ribosome biology to mechanisms underlying developmental phenotypes and tumor-associated translational reprogramming. RPL10A is therefore frequently studied in the context of ribosomopathies, lineage specification, and translation-dependent regulation of gene expression.
Ribosomal Protein L10a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RPL10A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RPL10A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RPL10A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RPL10A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.