Riboflavin kinase Antibody (E-7): sc-398830

Riboflavin kinase Antibody (E-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects Riboflavin kinase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Riboflavin kinase antibody (E-7) is available as the non-conjugated form. Riboflavin kinase, also known as RFK or RIFK, is a crucial cytoplasmic protein that plays a vital role in the biosynthesis of flavocoenzymes by catalyzing the ATP-dependent phosphorylation of riboflavin to produce flavin-mononucleotide (FMN), the first step in this pathway. RFK is predominantly expressed in the brain, placenta, and bladder, highlighting its importance in various physiological processes. The 162 amino acid structure of RFK is characterized by the presence of zinc and magnesium as essential cofactors, which are critical for enzymatic activity. RFK exhibits three distinct conformational states—namely the binary MgADP complex, the ternary product complex, and the apo form—each contributing to unique substrate binding and catalytic efficiency. Human RFK shares 44% homology with its yeast counterpart, indicating evolutionary conservation and suggesting that the flexible regions surrounding the active site, referred to as Flap I, Flap II, and Helix B, are structurally and functionally significant across species.

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Riboflavin kinase Antibody (E-7) References:

1. The riboflavin/FAD cycle in rat liver mitochondria.  |  Barile, M., et al. 2000. Eur J Biochem. 267: 4888-900. PMID: 10903524

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Riboflavin kinase Antibody (E-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Riboflavin kinase Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Riboflavin kinase Antibody (E-7) Specifications

Alternate Product Name: Riboflavin kinase

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 19-45

Epitope Target: Riboflavin kinase

Epitope Species: human

Product Reactivity/Detection: Riboflavin kinase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Riboflavin kinase Antibody (E-7): P: Blocking Peptide