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Rhodamine 6G (CAS 989-38-8)

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Alternate Names:
Basic Red 1
Application:
Rhodamine 6G is a rhodamine analog potentially useful in Pgp efflux assays
CAS Number:
989-38-8
Molecular Weight:
479.01
Molecular Formula:
C28H31N2O3Cl
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Rhodamine 6G, a fluorescent dye belonging to the rhodamine family, is extensively used in various scientific applications due to its intense fluorescence and good photostability. As a xanthene derivative, it is characterized by its bright orange-red fluorescence, which makes it a valuable tool in optical applications. Rhodamine 6G operates primarily through fluorescence mechanisms, where it absorbs light most efficiently at wavelengths around 530 nm and emits light at approximately 555 nm. This property is exploited in numerous research disciplines, particularly in fluorescence microscopy, where it is used to stain and visualize cells and subcellular components. Additionally, Rhodamine 6G serves a critical role in laser technologies; it is often employed as a laser dye in dye lasers where it acts as the active medium, providing laser light at specific wavelengths when excited by another light source. Its high quantum yield makes it an excellent choice for generating coherent, tunable laser output, which is useful in spectroscopy and photodynamic studies. In environmental science, Rhodamine 6G is utilized as a tracer dye to monitor water flow and pollutant dispersion due to its high solubility and distinct fluorescence, which allows for the easy tracking of water currents and dispersion patterns in complex aquatic systems. Through these diverse applications, Rhodamine 6G contributes significantly to advancements in both scientific research and practical technologies, underscoring its versatility and importance across various fields.


Rhodamine 6G (CAS 989-38-8) References

  1. The fluorescent cationic dye rhodamine 6G as a probe for membrane potential in bovine aortic endothelial cells.  |  Mandalà, M., et al. 1999. Anal Biochem. 274: 1-6. PMID: 10527490
  2. Absorption and fluorescence spectroscopy of rhodamine 6G in titanium dioxide nanocomposites.  |  Vogel, R., et al. 2004. Spectrochim Acta A Mol Biomol Spectrosc. 60: 245-9. PMID: 14670484
  3. The use of rhodamine 6G and fluorescence microscopy in the evaluation of phospholipid-based polymeric biomaterials.  |  Wang, JH., et al. 2005. J Microsc. 217: 216-24. PMID: 15725125
  4. Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy.  |  Eggeling, C., et al. 2005. Chemphyschem. 6: 791-804. PMID: 15884061
  5. Tunable cytotoxicity of rhodamine 6G via anion variations.  |  Magut, PK., et al. 2013. J Am Chem Soc. 135: 15873-9. PMID: 24059469
  6. Rhodamine 6G conjugated to gold nanoparticles as labels for both SERS and fluorescence
studies on live endothelial cells.  |  Jaworska, A., et al. 2015. Mikrochim Acta. 182: 119-127. PMID: 25568498
  7. Effect of Microemulsion Structure on Fluorescence and Nonlinear Optical Properties of Rhodamine 6G.  |  Pourtabrizi, M., et al. 2018. J Fluoresc. 28: 323-336. PMID: 29150729
  8. Rhodamine 6G Structural Changes in Water/Ethanol Mixed Solvent.  |  Chapman, M. and Euler, WB. 2018. J Fluoresc. 28: 1431-1437. PMID: 30343362
  9. Aggregation states of rhodamine 6G in mesostructured silica films  |  Malfatti L, Kidchob T, Aiello D, Aiello R, Testa F, Innocenzi P. 2008. The Journal of Physical Chemistry C. 112(42): 16225-16230.
  10. Trace determination of rhodamine B and rhodamine 6G dyes in aqueous samples by solid‐phase extraction and high‐performance liquid chromatography coupled with fluorescence detection  |  Chiang, T.L., Wang, Y.C. and Ding, W.H. 2012. Journal of the chinese chemical society. 59(4): 515-519.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rhodamine 6G, 25 g

sc-280066
25 g
$48.00

Rhodamine 6G, 100 g

sc-280066A
100 g
$138.00

Rhodamine 6G, 250 g

sc-280066B
250 g
$258.00

Rhodamine 6G, 1 kg

sc-280066C
1 kg
$790.00

Rhodamine 6G, 5 kg

sc-280066D
5 kg
$2859.00

What is the typical purity of Rhodamine 6G (CAS 989-38-8) (sc-280066)?

Asked by: Thomas Lee
Thank you for your question. Each lot of this chemical comes with a lot-specific Certificate Of Analysis which typically describes the percent purity as well as the method used to determine the purity. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to inquire about the purity of our most recent lot. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-08

Will there be any issue caused if I stored this compound at 5° C in a dry place?

Asked by: two2igm05
Thank you for your question. We suggest long term storage at -20º C. For short term storage, 5 ºC in a dry place should be sufficient.
Answered by: Technical Support
Date published: 2019-06-09
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Rated 5 out of 5 by from Benevides et alBenevides et al. (PubMed ID 26368112) analyzed the Rhodamine 6G dye degradation via fluorescence microscopy to elucidate degradation time behavior of fluorescent solutions. -SCBT Publication Review
Date published: 2015-02-02
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Rhodamine 6G is rated 5.0 out of 5 by 1.
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