Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RhoB Double Nickase Plasmid (h): sc-400875-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RhoB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RhoB Double Nickase Plasmid (h) and RhoB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RHOB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RhoB Antibody (C-5): sc-8048
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoB Double Nickase Plasmid (h) | sc-400875-NIC | 20 µg | $410.00 | |||
RhoB Double Nickase Plasmid (h2) | sc-400875-NIC-2 | 20 µg | $410.00 |
RHOB encodes the small GTPase RhoB, a membrane-associated regulator of actin dynamics and vesicular trafficking that cycles between GDP- and GTP-bound states to control cell shape, adhesion, and motility. RhoB integrates signals downstream of growth factor receptors and cellular stress pathways, influencing endosomal transport, receptor turnover, and cytoskeletal remodeling through Rho-family effector networks. It participates in processes such as migration, barrier function, and apoptotic or stress-adaptive responses, linking membrane trafficking to transcriptional and cytoskeletal outputs. Dysregulated RHOB signaling has been associated with altered invasive behavior and therapy stress responses in cancer models, as well as broader roles in vascular and inflammatory contexts.
RhoB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RHOB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RHOB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RHOB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RHOB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.