Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rho T2 CRISPR/Cas9 KO Plasmid (h): sc-406496
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rho T2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rho T2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rho T2 Antibody (E-9): sc-518198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rho T2 CRISPR/Cas9 KO Plasmid (h) | sc-406496 | 20 µg | $397.00 |
Overview
RHOT2 encodes Rho T2 (also known as Miro2), an atypical Rho-family GTPase anchored in the outer mitochondrial membrane that helps coordinate mitochondrial trafficking, positioning, and quality control. Through interactions with adaptor proteins linking mitochondria to microtubule motors, RHOT2 contributes to cytoskeletal-dependent transport and supports mitochondrial distribution required for energy-demanding cellular regions. Rho T2 is also implicated in calcium-sensitive regulation of mitochondrial dynamics and turnover, intersecting with pathways governing mitochondrial homeostasis and stress responses. Dysregulated RHOT2 function has been studied in the context of altered mitochondrial dynamics and bioenergetics, processes frequently associated with neurodegeneration and cancer-related metabolic reprogramming.
Rho T2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHOT2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHOT2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHOT2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rho T2 protein expression.
This CRISPR knockout system enables efficient generation of RHOT2-deficient cell models for investigation of Rho T2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHOT2 exon(s) critical for Rho T2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHOT2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rho T2 CRISPR/Cas9 KO Plasmid (h) and Rho T2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHOT2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rho T2 HDR Plasmid (h) and Rho T2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHOT2 homology arms to support homology-directed repair at defined RHOT2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.