RhoB Antibody (C-5): sc-8048
- RhoB Antibody (C-5) is a mouse monoclonal IgG1 κ RhoB antibody, cited in 65 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 123-137 within an internal region of Rho B of human origin
- RhoB Antibody (C-5) is recommended for detection of Rho B p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-RhoB Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for RhoB Antibody (C-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with RhoB Antibody (C-5) (see ordering information below).
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Rho B Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rho B of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Rho B antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rho B plays a crucial role in regulating the actin cytoskeleton, which is essential for various cellular processes such as cell migration, adhesion, and division. Proper Rho B function is vital for maintaining cellular integrity and facilitating communication between cells, particularly in the context of cancer progression where altered Rho B activity can lead to increased invasiveness and metastasis. Rho B is known to interact with several key proteins, including Rho-associated protein kinase (ROCK) and various guanine nucleotide exchange factors (GEFs), which further underscores Rho B importance in signal transduction pathways. A carboxy-terminal CAAX sequence in Rho B is critical for post-translational modification and membrane localization, ensuring correct positioning to exert effects within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RhoB Antibody (C-5) References:
- RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage. | Liu Ax,., et al. 2001. Proc Natl Acad Sci U S A. 98: 6192-7. PMID: 11353846
- RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells. | Liu, AX., et al. 2001. Mol Cell Biol. 21: 6906-12. PMID: 11564874
- RhoB controls the 24 kDa FGF-2-induced radioresistance in HeLa cells by preventing post-mitotic cell death. | Ader, I., et al. 2002. Oncogene. 21: 5998-6006. PMID: 12203112
- Androgen control of cell proliferation and cytoskeletal reorganization in human fibrosarcoma cells: role of RhoB signaling. | Chauhan, S., et al. 2004. J Biol Chem. 279: 937-44. PMID: 14576147
- EGFR, ErbB2 and Ras but not Src suppress RhoB expression while ectopic expression of RhoB antagonizes oncogene-mediated transformation. | Jiang, K., et al. 2004. Oncogene. 23: 1136-45. PMID: 14647415
- Akt mediates Ras downregulation of RhoB, a suppressor of transformation, invasion, and metastasis. | Jiang, K., et al. 2004. Mol Cell Biol. 24: 5565-76. PMID: 15169915
- Why three Rho proteins? RhoA, RhoB, RhoC, and cell motility. | Wheeler, AP. and Ridley, AJ. 2004. Exp Cell Res. 301: 43-9. PMID: 15501444
- RhoB and actin polymerization coordinate Src activation with endosome-mediated delivery to the membrane. | Sandilands, E., et al. 2004. Dev Cell. 7: 855-69. PMID: 15572128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoB Antibody (C-5) | sc-8048 | 200 µg/ml | $316.00 | |||
RhoB Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-531631 | 200 µg Ab; 20 µg BP | $354.00 | |||
RhoB Antibody (C-5) AC | sc-8048 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RhoB Antibody (C-5) HRP | sc-8048 HRP | 200 µg/ml | $316.00 | |||
RhoB Antibody (C-5) FITC | sc-8048 FITC | 200 µg/ml | $330.00 | |||
RhoB Antibody (C-5) PE | sc-8048 PE | 200 µg/ml | $343.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 488 | sc-8048 AF488 | 200 µg/ml | $357.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 546 | sc-8048 AF546 | 200 µg/ml | $357.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 594 | sc-8048 AF594 | 200 µg/ml | $357.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 647 | sc-8048 AF647 | 200 µg/ml | $357.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 680 | sc-8048 AF680 | 200 µg/ml | $357.00 | |||
RhoB Antibody (C-5) Alexa Fluor® 790 | sc-8048 AF790 | 200 µg/ml | $357.00 | |||
RhoB (C-5) Neutralizing Peptide | sc-8048 P | 100 µg/0.5 ml | $68.00 |