RHBDL6 Double Nickase Plasmid (h): sc-404212-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RHBDL6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RHBDL6 Double Nickase Plasmid (h) and RHBDL6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RHBDF2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RHBDL6 Double Nickase Plasmid (h) | sc-404212-NIC | 20 µg | $410.00 | |||
RHBDL6 Double Nickase Plasmid (h2) | sc-404212-NIC-2 | 20 µg | $410.00 |
RHBDF2 encodes an inactive rhomboid family member (iRhom2) that regulates trafficking and maturation of ADAM17/TACE, thereby controlling ectodomain shedding of multiple membrane proteins, including TNF and EGFR ligands. Through this role, RHBDF2 influences inflammatory signaling, EGFR pathway activation, and stress-responsive proteostasis in epithelial and immune contexts. Altered RHBDF2 activity has been linked to dysregulated cytokine and growth factor signaling and is associated with hyperproliferative and inflammatory phenotypes, making it relevant for mechanistic studies of skin biology, innate immunity, and signal-dependent cell fate decisions. RHBDL6 is commonly used as an alias in reagent naming, but the targeted locus here is the human RHBDF2 gene for pathway-centric functional genomics.
RHBDL6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RHBDF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RHBDF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RHBDF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RHBDF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.