RGS6 Double Nickase Plasmid (h): sc-404102-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RGS6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RGS6 Double Nickase Plasmid (h) and RGS6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RGS6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGS6 Double Nickase Plasmid (h) | sc-404102-NIC | 20 µg | $410.00 |
RGS6 (Regulator of G protein signaling 6) is a GTPase-activating protein that attenuates GPCR signaling by accelerating Gαi/o-mediated GTP hydrolysis, thereby shaping the amplitude and duration of heterotrimeric G protein pathways. Through modulation of second-messenger networks such as cAMP/PKA and downstream MAPK signaling, RGS6 contributes to control of cellular excitability, stress responses, and context-dependent survival signaling. RGS6 has been linked to regulation of dopaminergic and cholinergic signaling, and has been studied in processes relevant to neurobiology and cardiovascular physiology. Altered RGS6 expression or function has been associated with disease-relevant phenotypes including arrhythmogenic signaling, neuropsychiatric features, and cancer-associated pathways, supporting its value in mechanistic studies.
RGS6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RGS6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RGS6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RGS6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RGS6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.