RFLAT-1 Antibody (IP-2): sc-130454

RFLAT-1 Antibody (IP-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects RFLAT-1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-RFLAT-1 antibody (IP-2) is available as a non-conjugated format. RFLAT-1, also known as RANTES factor of late activated T lymphocytes-1, BTEB3, and Kruppel-like factor 13 (KLF13), serves as a crucial transcription factor predominantly expressed in T cells during later stages of activation. This delayed expression coincides with RANTES production, a key chemoattractant cytokine that plays a vital role in recruiting monocytes, T lymphocytes, eosinophils, basophils, and natural killer cells to sites of inflammation. RFLAT-1 is localized in the nucleus, where RFLAT-1 binds to the A site of the RANTES promoter, facilitating transcriptional activation. Structurally, RFLAT-1 relates to the TFIIA-like zinc finger protein superfamily, featuring three contiguous zinc finger motifs at its carboxy-terminus along with a proline-rich transcriptional activation domain, which are characteristic of TFIIA family proteins such as Sp1 and Sp3. RFLAT-1′s ability to activate gene-specific transcription in activated T cells is modulated by phosphorylation, and RFLAT-1′s late-stage transcriptional activation of RANTES is significantly influenced by Rel proteins from the NFκB family, indicating a potential synergistic relationship between RFLAT-1 and Rel proteins in regulating the RANTES promoter.

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RFLAT-1 Antibody (IP-2) References:

1. RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes.  |  Song, A., et al. 1999. Immunity. 10: 93-103. PMID: 10023774
2. Tight correlation between inhibition of DNA repair in vitro and transcription factor IIIA binding in a 5S ribosomal RNA gene.  |  Conconi, A., et al. 1999. EMBO J. 18: 1387-96. PMID: 10064604
3. Transcriptional regulation of T lymphocyte development and function.  |  Kuo, CT. and Leiden, JM. 1999. Annu Rev Immunol. 17: 149-87. PMID: 10358756
4. Kinetics of transcription factors regulating the RANTES chemokine gene reveal a developmental switch in nuclear events during T-lymphocyte maturation.  |  Ortiz, BD., et al. 1996. Mol Cell Biol. 16: 202-10. PMID: 8524297
5. Identification of a novel regulatory region critical for expression of the RANTES chemokine in activated T lymphocytes.  |  Nelson, PJ., et al. 1996. J Immunol. 157: 1139-48. PMID: 8757619
6. Switching gears during T-cell maturation: RANTES and late transcription.  |  Ortiz, BD., et al. 1997. Immunol Today. 18: 468-71. PMID: 9357137
7. RANTES and MIP-1alpha activate stats in T cells.  |  Wong, M. and Fish, EN. 1998. J Biol Chem. 273: 309-14. PMID: 9417081
8. Essential role of interferon regulatory factor 3 in direct activation of RANTES chemokine transcription.  |  Lin, R., et al. 1999. Mol Cell Biol. 19: 959-66. PMID: 9891032

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RFLAT-1 Antibody (IP-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RFLAT-1 Antibody (IP-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RFLAT-1 Antibody (IP-2) Specifications

Alternate Product Name: RFLAT-1

Product Clone Name: IP-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 31-72

Epitope Target: RFLAT-1

Epitope Species: human

Product Reactivity/Detection: RFLAT-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RFLAT-1 Antibody (IP-2): N/A