Date published: 2026-4-4

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Resorufin butyrate (CAS 15585-42-9)

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Alternate Names:
(7-oxophenoxazin-3-yl) Butanoate
Application:
Resorufin butyrate is a choline esterase and lipase fluorogenic substrate
CAS Number:
15585-42-9
Purity:
≥95%
Molecular Weight:
283.28
Molecular Formula:
C16H13NO4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Resorufin butyrate (RB) is a fluorescent dye employed in scientific research. It is derived from resorufin, a naturally occurring compound present in the red dye of certain plant species. With its distinct structure, Resorufin butyrate offers versatility for various laboratory applications, including cell imaging, protein labeling, and DNA sequencing. Moreover, Resorufin butyrate finds utility in biochemical and physiological investigations, such as the examination of cell signaling pathways and enzyme activity. Resorufin butyrate has garnered widespread use in scientific research due to its diverse range of applications. It serves as a valuable fluorescent label for proteins and DNA, facilitating their visualization and detection. Additionally, Resorufin butyrate has proven useful in biochemical and physiological studies, aiding in the exploration of cell signaling pathways and investigating enzyme functionality. Functioning as a fluorescent dye, Resorufin butyrate binds to its target molecule, triggering fluorescence activation. Conversely, the fluorescence intensity diminishes when Resorufin butyrate binds to the target molecule, leading to quenching. This fluorescence modulation phenomenon enables researchers to gain insights into molecular interactions and dynamic processes within biological systems. Moreover, Resorufin butyrate has found application in the evaluation of the effects of drugs and toxins on cellular function.


Resorufin butyrate (CAS 15585-42-9) References

  1. RESORUFIN BUTYRATE AND INDOXYL ACETATE AS FLUOROGENIC SUBSTRATES FOR CHOLINESTERASE.  |  GUILBAULT, GG. and KRAMER, DN. 1965. Anal Chem. 37: 120-3. PMID: 14230702
  2. Identification and characterization of a new true lipase isolated through metagenomic approach.  |  Glogauer, A., et al. 2011. Microb Cell Fact. 10: 54. PMID: 21762508
  3. Resorufin butyrate as a soluble and monomeric high-throughput substrate for a triglyceride lipase.  |  Lam, V., et al. 2012. J Biomol Screen. 17: 245-51. PMID: 21956174
  4. Mechanism of inhibition of Mycobacterium tuberculosis antigen 85 by ebselen.  |  Favrot, L., et al. 2013. Nat Commun. 4: 2748. PMID: 24193546
  5. Antibacterial activity of resazurin-based compounds against Neisseria gonorrhoeae in vitro and in vivo.  |  Schmitt, DM., et al. 2016. Int J Antimicrob Agents. 48: 367-72. PMID: 27451856
  6. Thermal and Photoinduced Copper-Promoted C-Se Bond Formation: Synthesis of 2-Alkyl-1,2-benzisoselenazol-3(2H)-ones and Evaluation against Mycobacterium tuberculosis.  |  Thanna, S., et al. 2017. J Org Chem. 82: 3844-3854. PMID: 28273423
  7. Exploring Covalent Allosteric Inhibition of Antigen 85C from Mycobacterium tuberculosis by Ebselen Derivatives.  |  Goins, CM., et al. 2017. ACS Infect Dis. 3: 378-387. PMID: 28285521
  8. Cyclipostins and cyclophostin analogs inhibit the antigen 85C from Mycobacterium tuberculosis both in vitro and in vivo.  |  Viljoen, A., et al. 2018. J Biol Chem. 293: 2755-2769. PMID: 29301937
  9. Mycolyltransferase from Mycobacterium tuberculosis in covalent complex with tetrahydrolipstatin provides insights into antigen 85 catalysis.  |  Goins, CM., et al. 2018. J Biol Chem. 293: 3651-3662. PMID: 29352107
  10. A lipoprotein lipase-GPI-anchored high-density lipoprotein-binding protein 1 fusion lowers triglycerides in mice: Implications for managing familial chylomicronemia syndrome.  |  Nimonkar, AV., et al. 2020. J Biol Chem. 295: 2900-2912. PMID: 31645434
  11. Biochemical and microbiological evaluation of N-aryl urea derivatives against mycobacteria and mycobacterial hydrolases.  |  Vartak, A., et al. 2019. Medchemcomm. 10: 1197-1204. PMID: 31741730
  12. A Fluorogenic Trehalose Probe for Tracking Phagocytosed Mycobacterium tuberculosis.  |  Dai, T., et al. 2020. J Am Chem Soc. 142: 15259-15264. PMID: 32813512
  13. Graphene oxide elicits microbiome-dependent type 2 immune responses via the aryl hydrocarbon receptor.  |  Peng, G., et al. 2023. Nat Nanotechnol. 18: 42-48. PMID: 36509925
  14. Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence.  |  Jenson, JM., et al. 2023. Nature. 616: 326-331. PMID: 36848932
  15. Reverse micelles as a water-property-control system to investigate the hydration/activity relationship of alpha-chymotrypsin.  |  Dorovska-Taran, V., et al. 1993. Eur J Biochem. 218: 1013-9. PMID: 8281919

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Resorufin butyrate, 25 mg

sc-208302
25 mg
$168.00

Resorufin butyrate, 100 mg

sc-208302A
100 mg
$490.00