Resolvin D1 is from a family of potent lipid mediators derived from both eicosapentaenoic acid (EPA) and docosahexaenoic (DHA)(1), it is an anti-inflammatory and promotes the resolution of the inflammatory response back to a non-inflamed state.2 RvD1 is produced physiologically from the sequential oxygenation of DHA by 15- and 5-lipoxygenase. It reduces human polymorphonuclear leukocyte (PMNL) transendothelial migration, the earliest event in acute inflammation, with EC50 values of ~30 nM. It exhibits a dose-dependent reduction in leukocyte infiltration in a murine model of peritonitis with a maximal inhibition of ~35% at a 10-100 ng dose.
A solution in ethanol
Soluble in water (0.05 mg/mL) at 25° C, PBS pH 7.2 (~0.05 mg/mL), DMSO (~50 mg/mL), DMF (~50 mg/mL), and ethanol.
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Hi, could the carboxyl group of resolvin D1 be further utilized in coupling reaction ? Is it stable for around 3 hours in water or DMF ?
Asked by: NickChen
The carboxyl group of Resolvin D1 can be utilized for conjugation chemistry under the proper conditions, although we do not provide a protocol for doing so.
Although we do not have formal stability data for item other than for the formulation in which it is supplied (EtOH solution), based on the 24 hour (at 4 degree C) stability of the standard curve of our Resolvin D1 ELISA, which is an aqueous solution, it would likely be stable for 3 hours in water or DMF (although we cannot provide a guarantee without data to confirm).
Answered by: Technical Support
Date published: 2019-01-26
How long can I store dissolved RvD1?
Asked by: Jz28sail
Thank you for your question.
Resolvin D1, sc-204877, is supplied in a solution of ethanol. It is stable as supplied at -80°C. Resolvins can isomerize and degrade when put into freeze thaw conditions or in solvents such as dimethyl formamide or DMSO. The compound should be used immediately when dissolved in an aqueous buffer.
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