Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rent1 Double Nickase Plasmid (h): sc-402462-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rent1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rent1 Double Nickase Plasmid (h) and Rent1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UPF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rent1 Antibody (C-6): sc-393594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rent1 Double Nickase Plasmid (h) | sc-402462-NIC | 20 µg | $410.00 | |||
Rent1 Double Nickase Plasmid (h2) | sc-402462-NIC-2 | 20 µg | $410.00 |
UPF1 (Rent1) encodes an RNA helicase/ATPase that is central to nonsense-mediated mRNA decay (NMD), a conserved RNA surveillance pathway that detects premature termination codons and promotes decay of aberrant transcripts. By coordinating with translation termination factors and NMD components such as SMG1, SMG5/6/7, UPF2, and UPF3, UPF1 regulates transcriptome quality control, mRNA turnover, and proteostasis. UPF1 activity also intersects with DNA damage responses, telomere maintenance, and antiviral RNA restriction, linking RNA metabolism to broader cellular stress pathways. Dysregulation of NMD and UPF1-dependent RNA processing has been associated with neurodevelopmental phenotypes, tumor biology, and viral infection studies, making UPF1 a widely used target for mechanistic investigations in human cells.
Rent1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UPF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UPF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UPF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UPF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.