Reg IIIα CRISPR Activation Plasmid (h): sc-404278-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Reg IIIα CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Reg IIIα CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Reg IIIα CRISPR Activation Plasmid (h) and Reg IIIα CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the REG3A transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Reg IIIα CRISPR Activation Plasmid (h) | sc-404278-ACT | 20 µg | $397.00 |
REG3A encodes the secreted C-type lectin Reg IIIα, an antimicrobial protein prominently expressed by intestinal and pancreatic epithelial cells where it contributes to mucosal barrier integrity and innate immune defense. Reg IIIα binds bacterial peptidoglycan and helps shape host–microbiome interactions, linking epithelial stress responses to microbial clearance. Its expression is regulated by cytokine-driven pathways such as IL-22/STAT3 and is frequently induced during inflammation and tissue injury, supporting epithelial restitution and remodeling programs. Dysregulated REG3A/Reg IIIα activity has been associated with inflammatory bowel disease, pancreatitis, and colorectal cancer–related inflammation, making it a useful marker and functional node in studies of epithelial immunity.
Reg IIIα CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous REG3A expression without altering the underlying DNA sequence.
Reg IIIα CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the REG3A locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the REG3A transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Reg IIIα expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native REG3A locus and enabling the study of Reg IIIα-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Reg IIIα pathway restoration in tumor cells with silenced or reduced REG3A expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.