Recombinant Protein G Agarose
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Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol). It is used for the purification of IgG from most mammalian species, and does not bind IgM, IgA, IgE, or serum albumin. The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads. rProtein G Agarose has a binding capacity of approximately 20 mg of human IgG per mL of beads. rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A. The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography.
Binding of recombinant Protein G and Protein A to various IgG types:
| Species | rProtein G | Protein A |
|---|---|---|
| Goat | ++ | - |
| Sheep | ++ | - |
| Horse | ++ | - |
| Cow | ++ | - |
| Rat | + | - |
| Mouse | ++ | + |
| Human | ++ | ++ |
| Rabbit | ++ | ++ |
| Guinea Pig | ++ | ++ |
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Recombinant Protein G Agarose, 5 ml | sc-500780 | 5 ml | $906.00 |