Rb Antibody (H-2): sc-74570

Rb Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 769-921 at the C-terminus of mouse Rb, a crucial tumor suppressor involved in cell cycle regulation. Rb mouse monoclonal antibody (H-2) recognizes an epitope within the specified immunogen sequence and demonstrates broad species cross-reactivity, detecting Rb in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. The retinoblastoma protein functions as a sophisticated regulator of cell cycle progression, particularly at the G1/S transition, through interactions with E2F transcription factors. In hypophosphorylated form, Rb actively represses genes required for S phase entry by binding to E2F factors, establishing a crucial checkpoint against uncontrolled cell proliferation. This repression undergoes precise regulation during G1 phase, when cyclin D-dependent kinases phosphorylate Rb at multiple serine and threonine residues, triggering Rb inactivation and E2F release to permit cell cycle advancement. Beyond direct transcriptional control, Rb enhances gene silencing by recruiting histone deacetylases to target promoter regions, promoting nucleosome formation and masking transcription-enhancing elements. Mutations disrupting Rb function can lead to various human cancers, including retinoblastoma, osteosarcoma, small cell lung carcinoma, and breast cancer. Additionally, viral oncoproteins such as HPV E7 can bind and inactivate Rb, highlighting Rb′s significance in both conventional and viral-mediated carcinogenesis. Through these diverse cellular functions, Rb (H-2) antibody serves as a valuable tool for investigating cell cycle regulation mechanisms and cancer development pathways.

The preferred Rb antibody is Rb Antibody (IF8): sc-102.