RAW 264.7 + PMA nuclear extract: sc-24962
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- mouse nuclear extract; Abelson transformed macrophage cells induced with PMA
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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SEE ALSO...
The RAW 264.7 + PMA nuclear extract is derived from RAW 264.7 cells, a murine macrophage cell line, stimulated with phorbol 12-myristate 13-acetate (PMA). This treatment activates numerous signaling pathways, leading to the differentiation and activation of these macrophage cells. Specifically, PMA is known to activate protein kinase C (PKC), which plays a pivotal role in the transcriptional regulation of genes involved in immune response and cell adhesion. The nuclear extract from these cells is particularly rich in transcription factors and other nuclear proteins that are critical for studying gene expression and nuclear signaling pathways. Research applications of this nuclear extract include examining the dynamics of chromatin remodeling, the interaction between transcription factors and DNA, and the cellular response to external stimuli at the nuclear level. This tool is invaluable in basic science for dissecting the mechanisms of macrophage activation and the regulation of gene expression. It provides a complex mixture of proteins that can be studied to understand the transcriptional responses induced by PKC activation in a model macrophage system.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAW 264.7 + PMA nuclear extract | sc-24962 | 250 µg/0.05 ml | $160.00 |