rat heart extract: sc-2393
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- Western blotting positive control
- recommended use is 50 µg (20 µl) per lane
- extracts should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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Rat heart extract, derived from rat heart tissue, serves as a valuable research tool in scientific investigations. This complex lysate contains proteins, enzymes, nucleic acids, and metabolites. Researchers utilize rat heart extract to study cardiac physiology and pathology. It has been extensively used to explore molecular mechanisms associated with cardiac development, contractility, and metabolism. The extract enables investigations into cellular responses, signaling pathways, and the effects of growth factors, hormones, and stressors on cardiac cells. Through rat heart extract, researchers have explaind signaling cascades involved in cardiac hypertrophy, apoptosis, and regeneration. The lysate facilitates assessments of protein expression and enzyme activity, as well as metabolic adaptations and energy utilization. Through the use of rat heart extract, researchers have explaind complex signaling cascades involved in cardiac hypertrophy, apoptosis, and regeneration. The lysate has proven valuable in assessing the expression levels and activity of specific proteins and enzymes implicated in these processes. Furthermore, the extract has facilitated investigations into the metabolic adaptations of cardiac cells under different conditions, shedding light on energy utilization, substrate preferences, and metabolic reprogramming.
rat heart extract References:
- Decreased expression of high-molecular-weight calmodulin-binding protein and its correlation with apoptosis in ischemia-reperfused rat heart. | Kakkar, R., et al. 2001. Cell Calcium. 29: 59-71. PMID: 11133356
- Activation of calcineurin expression in ischemia-reperfused rat heart and in human ischemic myocardium. | Lakshmikuttyamma, A., et al. 2003. J Cell Biochem. 90: 987-97. PMID: 14624458
- Characterization of natural vasostatin-containing peptides in rat heart. | Glattard, E., et al. 2006. FEBS J. 273: 3311-21. PMID: 16857014
- The large isoforms of A-kinase anchoring protein 18 mediate the phosphorylation of inhibitor-1 by protein kinase A and the inhibition of protein phosphatase 1 activity. | Singh, A., et al. 2011. Mol Pharmacol. 79: 533-40. PMID: 21149637
- Radioimmunoassay for testicular cytochrome c (ct). Evidence for the presence of apocytochrome ct pool in rat testis extract. | Kim, IC. 1987. J Biol Chem. 262: 11156-62. PMID: 2440885
- Exosomes Derived From Mesenchymal Stem Cells Pretreated With Ischemic Rat Heart Extracts Promote Angiogenesis via the Delivery of DMBT1. | Xiao, Y., et al. 2022. Cell Transplant. 31: 9636897221102898. PMID: 35726847
- Purification and characterization of hormone-regulated isoforms of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovaries. | Jahnsen, T., et al. 1985. J Biol Chem. 260: 15980-7. PMID: 3934166
- Leucocyte migration inhibition by heart extract and liver mitochondria in patients with myocardial infarction. | Wartenberg, J. and Brostoff, J. 1973. Br Heart J. 35: 845-8. PMID: 4729841
- An effect of diet on the activity of phosphofructokinase in rat heart. | Clark, MG., et al. 1982. Biochem Biophys Res Commun. 105: 44-50. PMID: 6212056
- Adenine nucleotide metabolism and compartmentalization in isolated adult rat heart cells. | Geisbuhler, T., et al. 1984. Circ Res. 54: 536-46. PMID: 6373048
- Hormonal regulation of the phosphorylation of glycogen synthase in perfused rat heart. Effects of insulin, catecholamines, and glucagon. | Ramachandran, C., et al. 1983. J Biol Chem. 258: 13377-83. PMID: 6415063
- Dephosphorylation of glycogen synthase in rat heart extracts by E. coli alkaline phosphatase. Use of an exogenous phosphatase to study substrate-mediated regulation of dephosphorylation. | Lau, KH., et al. 1982. Mol Cell Biochem. 44: 149-59. PMID: 6810091
- Oxygen dependence of dopamine-beta-hydoxylase activity and lactate metabolism in synaptosomes from rat brain. | Katz, IR. 1982. Brain Res. 231: 399-409. PMID: 7055686
- Biochemical and enzymological study of lactate dehydrogenase isoenzymes from commercial quality control sera and several animal tissue sources. | Tanishima, K., et al. 1995. Eur J Clin Chem Clin Biochem. 33: 865-8. PMID: 8620065
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rat heart extract | sc-2393 | 500 µg/200 µl | $120.00 |