RASSF1 Antibody (3F3): sc-58470

RASSF1 Antibody (3F3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RASSF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-RASSF1 antibody (3F3) is available in a non-conjugated form, allowing for versatile experimental use. The Ras association domain family 1 (RASSF1) protein plays a crucial role in cellular signaling pathways, particularly in the regulation of cell death and tumor suppression. Located at the human lung tumor suppressor locus 3p21.3, RASSF1 consists of two major isoforms, RASSF1A and RASSF1C, which exhibit distinct functions in cellular processes. RASSF1A interacts with activated Ras proteins in a GTP-dependent manner, mediating the apoptotic effects of oncogenic Ras and thereby influencing cell fate decisions. RASSF1 epigenetic inactivation through hypermethylation occurs in various cancers, including lung and breast cancer, leading to a loss of tumor suppressor functions. Mutant forms of RASSF1A have shown significantly reduced growth suppression activity, highlighting RASSF1′s potential as a critical player in tumor pathogenesis. RASSF1 monoclonal antibody (3F3) enables researchers to gain valuable insights into cancer development mechanisms and RASSF1′s role in cellular regulation.

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RASSF1 Antibody (3F3) References:

1. Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3.  |  Dammann, R., et al. 2000. Nat Genet. 25: 315-9. PMID: 10888881
2. Ras uses the novel tumor suppressor RASSF1 as an effector to mediate apoptosis.  |  Vos, MD., et al. 2000. J Biol Chem. 275: 35669-72. PMID: 10998413
3. Methylation associated inactivation of RASSF1A from region 3p21.3 in lung, breast and ovarian tumours.  |  Agathanggelou, A., et al. 2001. Oncogene. 20: 1509-18. PMID: 11313894
4. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression.  |  Burbee, DG., et al. 2001. J Natl Cancer Inst. 93: 691-9. PMID: 11333291
5. The candidate tumor suppressor gene, RASSF1A, from human chromosome 3p21.3 is involved in kidney tumorigenesis.  |  Dreijerink, K., et al. 2001. Proc Natl Acad Sci U S A. 98: 7504-9. PMID: 11390984
6. The CpG island of the novel tumor suppressor gene RASSF1A is intensely methylated in primary small cell lung carcinomas.  |  Dammann, R., et al. 2001. Oncogene. 20: 3563-7. PMID: 11429703
7. RASSF1 tumor suppressor gene in pancreatic ductal adenocarcinoma: correlation of expression, chromosomal status and epigenetic changes.  |  Amato, E., et al. 2016. BMC Cancer. 16: 11. PMID: 26754001
8. EGFL7 and RASSF1 promoter hypermethylation in epithelial ovarian cancer.  |  Rattanapan, Y., et al. 2018. Cancer Genet. 224-225: 37-40. PMID: 29778234
9. RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice.  |  Guo, M., et al. 2019. Cell Biol Int. 43: 1163-1173. PMID: 30571844
10. Hypermethylated RASSF1 and SLC5A8 promoters alongside BRAFV600E mutation as biomarkers for papillary thyroid carcinoma.  |  Khatami, F., et al. 2020. J Cell Physiol. 235: 6954-6968. PMID: 32017063
11. The Role of RASSF1 Methylation in Lung Carcinoma.  |  Zhang, T., et al. 2020. Adv Exp Med Biol. 1255: 99-108. PMID: 32949393
12. RASSF1 is identified by transcriptome coordination analysis as a target of ATF4.  |  Zhang, Y., et al. 2023. FEBS Open Bio. 13: 556-569. PMID: 36723232

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RASSF1 Antibody (3F3) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-RASSF1 Antibody (3F3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RASSF1 Antibody (3F3) Specifications

Alternate Product Name: RASSF1

Product Clone Name: 3F3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-340

Epitope Target: RASSF1

Epitope Species: human

Product Reactivity/Detection: RASSF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for RASSF1 Antibody (3F3): N/A

The preferred RASSF1 antibody is RASSF1 Antibody (A-2): sc-515949.