RAR alpha Antibody (C-1) recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA

RARα Antibody (C-1): sc-515796

RARα Antibody (C-1) is rated 4.7 out of 5 by 9.
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Datasheets

    • Anti-RARα Antibody (C-1) is a mouse monoclonal IgG1 (kappa light chain) RARα antibody provided at 200 µg/ml
    • raised against amino acids 63-362 mapping within an internal region of RARα of human origin
    • Anti-RAR alpha Antibody (C-1) is recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-RAR alpha Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RARα (C-1): sc-515796.
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I used this on mice liver tissue total proteins western blot but I get two bands around 52 and another band around 37. I would like to know if there is anything to fix it?

Asked by: fathi21
Thank you for your question. The expected molecular weight for this protein is ~52kDa, but we do not have any information regarding a lower sized band. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-05-20

I would like to use this antibody for IHC(p) using DAB. May you recommend a secundary antibody?

Asked by: cristiane pereira
Thank you for your question. This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time. RARα Antibody (C-1) is a mouse monoclonal IgG1. For IHC(P), we recommend using a secondary antibody that is anti-mouse IgG or anti-mouse IgG1. For immunoperoxidase staining with DAB as the substrate, we would recommend using either our Mouse IgG Binding Protein conjugated to HRP, m-IgGκ BP-HRP: sc-516102, or our Mouse IgG Binding Protein conjugated to Biotin, m-IgGκ BP-B: sc-516142 in combination with our ImmunoCruz® ABC Kit: sc-516216 for signal amplification.
Answered by: Technical Support
Date published: 2019-04-25

Can this antibody be used for ChiP assays in mouse? Is there a reference for the same? Do I need to order a more concentrated form of the antibody for that purpose?

Asked by: Namr
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP.
Answered by: Technical Support
Date published: 2019-04-20

Can RARα Antibody ( (C-1): sc-515796) be used in  immunohistochemistry staining of human tissue 

Asked by: xueju
Thank you for your question. We have not yet tested this mouse monoclonal antibody, RARα (C-1): sc-515796 on formalin-fixed paraffin-embedded tissues and so this application is not recommended at this time. We do warranty the antibody for use in IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. However, we do not yet have any data in this application to show on our website. As soon as IHC or IF data is available, we will publish it on our website. We do currently offer one other antibody to this target, RARα (1C10): sc-293417. However, this antibody has also not yet been tested for immunohistochemistry. For a limited time, we are offering a free 10µg sample of RARα (C-1): sc-515796. If you would like to request a sample for testing in your application, please contact our Technical Service Department. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-15

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if RARα (C-1): sc-515796 mouse monoclonal antibody will work?

Asked by: Trav11
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01

RARa antibody(C-1) also can recognize RAR beta or RAR gamma?

Asked by: rere
Thank you for your question. RARα Antibody (C-1): sc-515796 is a mouse monoclonal antibody that is specific for RARα of mouse, rat and human origin. It is not expected to cross-react with RARβ or RARγ.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 5 out of 5 by from Dr Sadek Ab work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 5 out of 5 by from Excellent antibody This antibody works very well with minimal background.
Date published: 2018-05-02
Rated 4 out of 5 by from 反応性がよい。 一次抗体は1000倍に希釈して用いましたが、反応性がよく、しっかりとしたバンドが特異的に検出されましたので引き続き使用したいと考えております。
Date published: 2018-02-02
Rated 5 out of 5 by from Quite good for wb of rat protein 1:500 diluted for wb of rat protein ,the band is strong and clear!
Date published: 2017-09-27
Rated 5 out of 5 by from Great! I received this as a free sample. I used it at a 1:1000 dilution, and the secondary GAM at 1:1000 dilution on mouse and rat liver homogenates that were run on a 8% gel transferred to nitrocellulose membrane. The antibody was very clean with very little background.
Date published: 2017-09-26
Rated 5 out of 5 by from a good antibody for WB I used this antibody to check my macrophage sample, it works very good
Date published: 2017-09-22
Rated 4 out of 5 by from Good antibody The antibody works great at 1:500 dilution for WB and 1:100 in IF.
Date published: 2017-09-11
Rated 4 out of 5 by from great product I applied for the trial application of the product the day before yesterday.i received this antibody yesterday,so i use it to do the experiment .the result of western blot shows that the strip is clear , but multiple bands can be seen,it showed in the picture The condition of running glue is 140v, 55min, using 0.45 micron pore size NC film, constant pressure 100v, 1h40min. Then 5% skim milk closed for 1 h, 5% BSA by 1: 1000 ratio plus primary antibody (the sample), 4 degrees overnight, TBST washed 3 times, and then incubated with fluorescent secondary antibody 1h.
Date published: 2017-09-08
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