rapsyn Antibody (1234): sc-58585

rapsyn Antibody (1234) is a mouse monoclonal IgG1 antibody that detects rapsyn protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-rapsyn antibody (1234) is available as a non-conjugated format. The RAPSN gene locus is located on chromosome 11p11.1-p11.2 and encodes a peripheral membrane protein that plays a crucial role in neuromuscular transmission. Rapsyn, or receptor-associated protein of the synapse, is primarily expressed in the postsynaptic membrane of skeletal muscle, where rapsyn is essential for the clustering of nicotinic acetylcholine receptors (nAChR). Rapsyn′s ability to self-associate through tetra-tricopeptide repeats facilitates interaction with the large intracellular domain of the nAChR α subunit, which is vital for proper localization and function of these receptors at the neuromuscular junction. Rapsyn not only modifies AChR trafficking within the cell but is also critical for agrin-induced AChR clustering, a process essential for functional synapse formation. Notably, absence of rapsyn leads to deficits in postsynaptic specialization formation, resulting in increased axonal branching and motoneuron survival, highlighting rapsyn′s importance in neuromuscular health and signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

rapsyn Antibody (1234) References:

1. The intracellular domain of the nicotinic acetylcholine receptor alpha subunit mediates its coclustering with rapsyn.  |  Maimone, MM. and Enigk, RE. 1999. Mol Cell Neurosci. 14: 340-54. PMID: 10588389
2. Overexpression of rapsyn modifies the intracellular trafficking of acetylcholine receptors.  |  Han, H., et al. 2000. J Neurosci Res. 60: 155-63. PMID: 10740220
3. Overexpression of rapsyn inhibits agrin-induced acetylcholine receptor clustering in muscle cells.  |  Han, H., et al. 1999. J Neurocytol. 28: 763-75. PMID: 10859577
4. Promotion of motoneuron survival and branching in rapsyn-deficient mice.  |  Banks, GB., et al. 2001. J Comp Neurol. 429: 156-65. PMID: 11086296
5. Role of rapsyn tetratricopeptide repeat and coiled-coil domains in self-association and nicotinic acetylcholine receptor clustering.  |  Ramarao, MK., et al. 2001. J Biol Chem. 276: 7475-83. PMID: 11087759
6. The postsynaptic submembrane machinery at the neuromuscular junction: requirement for rapsyn and the utrophin/dystrophin-associated complex.  |  Banks, GB., et al. 2003. J Neurocytol. 32: 709-26. PMID: 15034263
7. Rapsyn as a signaling and scaffolding molecule in neuromuscular junction formation and maintenance.  |  Xing, G., et al. 2020. Neurosci Lett. 731: 135013. PMID: 32344108
8. The role of Rapsyn in neuromuscular junction and congenital myasthenic syndrome.  |  Liao, X., et al. 2023. Biomol Biomed. 23: 772-784. PMID: 36815443
9. Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN.  |  Sun, Y., et al. 2024. J Nanobiotechnology. 22: 247. PMID: 38741123
10. Cloning of cDNA encoding human rapsyn and mapping of the RAPSN gene locus to chromosome 11p11.2-p11.1.  |  Buckel, A., et al. 1996. Genomics. 35: 613-6. PMID: 8812503

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-rapsyn Antibody (1234) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-rapsyn Antibody (1234) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

rapsyn Antibody (1234) Specifications

Alternate Product Name: rapsyn

Product Clone Name: 1234

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: rapsyn

Epitope Species: Torpedo californica

Product Reactivity/Detection: rapsyn

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for rapsyn Antibody (1234): N/A