Rap1GAP Antibody (F-5): sc-166587

Rap1GAP Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects Rap1GAP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rap1GAP plays a crucial role in cellular signaling by specifically stimulating the GTP hydrolytic activity of the monomeric G protein Rap1, which is essential for regulating various cellular processes, including cell proliferation and differentiation. Rap1GAP is predominantly located in the brain, kidney, and pancreas, where Rap1GAP acts as a signal integrator, coordinating Gz signaling and Rap1 signaling pathways. Notably, Rap1GAP interacts with Gα z, a member of the Gi family of trimeric G proteins, which inhibits the ability of regulators of G protein signaling to promote GTP hydrolysis of the α subunit, thereby modulating downstream signaling events. Additionally, a novel isoform known as Rap1GAPII has been identified, which specifically binds to Gα z and is translocated from the cytosol to the membrane upon stimulation of the Gi-coupled M2 Muscarinic receptor, leading to a decrease in GTP-bound Rap1 and subsequent activation of the ERK/MAPK pathway. anti-Rap1GAP antibody (F-5) versatility in various applications makes this reagent an invaluable tool for researchers studying the intricate mechanisms of cellular signaling and the role of Rap1GAP in health and disease.

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Rap1GAP Antibody (F-5) References:

1. Rap1GAP inhibits tumor growth in oropharyngeal squamous cell carcinoma.  |  Zhang, Z., et al. 2006. Am J Pathol. 168: 585-96. PMID: 16436672
2. Downregulation of Rap1GAP contributes to Ras transformation.  |  Tsygankova, OM., et al. 2007. Mol Cell Biol. 27: 6647-58. PMID: 17646383
3. Downregulation of Rap1GAP in human tumor cells alters cell/matrix and cell/cell adhesion.  |  Tsygankova, OM., et al. 2010. Mol Cell Biol. 30: 3262-74. PMID: 20439492
4. Rap1GAP interacts with RET and suppresses GDNF-induced neurite outgrowth.  |  Jiao, L., et al. 2011. Cell Res. 21: 327-37. PMID: 20877310
5. Rap1GAP alters leukemia cell differentiation, apoptosis and invasion in vitro.  |  Qiu, T., et al. 2012. Oncol Rep. 28: 622-8. PMID: 22614916
6. RAP1GAP inhibits cytoskeletal remodeling and motility in thyroid cancer cells.  |  Dong, X., et al. 2012. Endocr Relat Cancer. 19: 575-88. PMID: 22696507
7. PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation.  |  Wang, D., et al. 2014. PLoS One. 9: e110296. PMID: 25329897
8. Low expression of Rap1GAP is associated with epithelial-mesenchymal transition (EMT) and poor prognosis in gastric cancer.  |  Yang, Y., et al. 2017. Oncotarget. 8: 8057-8068. PMID: 28009991
9. The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells.  |  Wang, Y., et al. 2021. Infect Agent Cancer. 16: 71. PMID: 34952616
10. CpG Island Methylation of the Rap1Gap Gene in Medullary Thyroid Cancer.  |  Faam, B., et al. 2022. Arch Iran Med. 25: 171-177. PMID: 35429959

The preferred Rap1GAP antibody is Rap1GAP Antibody (D-9): sc-166586.