Each CRISPR/Cas9 Knockout (KO) Plasmid product consists of a pool of 3 plasmids designed to ensure identification and cleavage of a specific gene for maximum knockout efficiency. Each plasmid encodes a unique 20 nt sequence derived from the GeCKO (v2) library.
Rap 1A CRISPR Plasmids (h)
- Target species: human; for corresponding mouse product, see Rap 1A CRISPR Plasmids (m)
- CRISPR/Cas9 KO Plasmids consists of Rap 1A-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using Rap 1A Antibody (C-10): sc-373968
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
CRISPR Activation Plasmids
Recommended Control Products
SEE ALSO...
Rap 1A Antibodies for analysis of cellular responses to Rap 1A CRISPR Products
Learn more about the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details
Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rap 1A CRISPR/Cas9 Knockout (KO) Plasmid (h) consists of a pool of three plasmids each encoding the Cas9 nuclease and a Rap 1A-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
- gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- **** NOTE: No corresponding HDR is available for this Knockout Plasmid. **** Please use sc-400572-KO-2 and sc-400572-HDR-2 if selection of cells using puromycin resistance is desired.
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rap 1A Antibody (C-10): sc-373968
See Protocol Online.
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Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rap 1A CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Rap 1A CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: Rap 1A Antibody (C-10): sc-373968
See Protocol Online.
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Related Products
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rap 1A CRISPR/Cas9 KO Plasmid (h) | sc-400572 | 20 µg | $397.00 | |||
Rap 1A CRISPR Activation Plasmid (h) | sc-400572-ACT | 20 µg | $397.00 | |||
Rap 1A CRISPR Activation Plasmid (h2) | sc-400572-ACT-2 | 20 µg | $397.00 |