Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ran CRISPR/Cas9 KO Plasmid (m): sc-422594
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ran CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ran genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ran Antibody (A-7): sc-271376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ran CRISPR/Cas9 KO Plasmid (m) | sc-422594 | 20 µg | $397.00 |
Overview
Ran (Ras-related nuclear protein) is a conserved small GTPase that establishes a RanGTP/RanGDP gradient across the nuclear envelope to drive directional nucleocytoplasmic transport through importin/exportin pathways. Beyond nuclear import and export, Ran regulates mitotic spindle assembly, centrosome function, and reformation of the nuclear envelope, coupling transport dynamics to cell-cycle progression. Through these roles, Ran influences transcriptional regulation, genome stability, and stress responses that are frequently perturbed in proliferative and neurodevelopmental disorders and cancer-associated cellular phenotypes. In mouse systems, Ran is widely used to interrogate core mechanisms of mitosis and nuclear transport that shape differentiation and tissue homeostasis.
Ran CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ran gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ran together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ran open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ran protein expression.
This CRISPR knockout system enables efficient generation of Ran-deficient cell models for investigation of Ran signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ran exon(s) critical for Ran function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ran genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ran CRISPR/Cas9 KO Plasmid (m) and Ran CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ran locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ran HDR Plasmid (m) and Ran HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ran homology arms to support homology-directed repair at defined Ran target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.