Ramos nuclear extract: sc-2153
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; Burkitt's lymphoma cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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The Ramos nuclear extract is prepared from Ramos cells, a human B lymphocyte cell line derived from a Burkitt's lymphoma. This cell line serves as a key model for studying B cell biology, specifically focusing on mechanisms such as B cell activation, receptor signaling, and apoptotic pathways. The nuclear extract from Ramos cells is enriched with nuclear proteins including transcription factors, coactivators, and chromatin-modifying enzymes, which are crucial for studying the regulation of gene expression linked to B cell development and function. This extract has been extensively used in research to dissect the nuclear signaling pathways that govern the immune response and cell cycle progression in B cells. For instance, it facilitates the analysis of NF-kB signaling dynamicsa pathway fundamental to immune responses and inflammationas well as the study of mechanisms regulating antibody production. These applications are instrumental in understanding the intrinsic cellular processes in B lymphocytes, offering insights into the regulation of gene transcription. This makes Ramos nuclear extract a valuable tool for fundamental immunological research.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ramos nuclear extract | sc-2153 | 250 µg/0.05 ml | $160.00 |