Ramiprilat-d3 (CAS 1356933-70-4)

Ramiprilat-d3 is a deuterated analog of ramiprilat, the active metabolite of ramipril, where three hydrogen atoms have been replaced by deuterium. This modification is primarily utilized in analytical chemistry, particularly in mass spectrometry, to serve as an internal standard for the precise quantification of ramiprilat and its metabolites in biological samples. The deuteration imparts a slight mass difference without significantly altering the chemical properties of the molecule, which allows researchers to distinguish between the labeled and unlabeled compounds in complex mixtures. In research, Ramiprilat-d3 is valuable for studying the biotransformation of ramiprilat, providing insights into the metabolic pathways and enzyme kinetics involved in the conversion of ramipril to its active form. The incorporation of deuterium can also slightly influence the rate of metabolic reactions, offering a tool to investigate isotope effects on enzyme-substrate interactions, particularly within the context of angiotensin-converting enzyme (ACE) activity, which plays a critical role in regulating cardiovascular and renal functions through the conversion of angiotensin I to angiotensin II.