BRAF Antibody (13): sc-136263
- BRAF Antibody (13) is a mouse monoclonal IgG2a, cited in 2 publications, provided at 50 µg/0.5 ml
- raised against amino acids 285-406 of Raf-B of human origin
- recommended for detection of Raf-B of mouse, rat and human origin by WBIP
- See BRAF (F-7): sc-5284 for BRAF antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for BRAF Antibody (13). This work is in progress.
Raf-B Antibody (13) is a mouse monoclonal IgG2a antibody that detects Raf-B in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Raf-B is a member of the Raf gene family of serine/threonine protein kinases, which play a crucial role in the regulation of cell signaling pathways that control various cellular processes, including growth, differentiation, and survival. Raf-B is primarily located in the cytoplasm, where anti-Raf-B antibody (13) identifies this key mediator in the mitogen-activated protein kinase (MAPK) signaling cascade, influencing cellular responses to growth factors and stress signals. Understanding Raf-B localization is important because cellular activity can be modulated by various upstream signals, and misregulation has been implicated in several diseases, including cancer. Raf-B exhibits a high degree of homology with Raf-1, particularly within the kinase domain, suggesting shared regulatory mechanisms and functional roles in cellular signaling. Raf-B (13) monoclonal antibody serves as an essential tool for researchers studying the intricate signaling networks that govern cell behavior and potential therapeutic targets within these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRAF Antibody (13) References:
- Isotype-specific functions of Raf kinases. | Hagemann, C. and Rapp, UR. 1999. Exp Cell Res. 253: 34-46. PMID: 10579909
- Conferring specificity on the ubiquitous Raf/MEK signalling pathway. | O'Neill, E. and Kolch, W. 2004. Br J Cancer. 90: 283-8. PMID: 14735164
- Mechanism of activation of the RAF-ERK signaling pathway by oncogenic mutations of B-RAF. | Wan, PT., et al. 2004. Cell. 116: 855-67. PMID: 15035987
- B-Raf specific antibody responses in melanoma patients. | Fensterle, J., et al. 2004. BMC Cancer. 4: 62. PMID: 15361259
- Functional analysis of the regulatory requirements of B-Raf and the B-Raf(V600E) oncoprotein. | Brummer, T., et al. 2006. Oncogene. 25: 6262-76. PMID: 16702958
- Discovery of a selective inhibitor of oncogenic B-Raf kinase with potent antimelanoma activity. | Tsai, J., et al. 2008. Proc Natl Acad Sci U S A. 105: 3041-6. PMID: 18287029
- Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling. | Röring, M., et al. 2012. EMBO J. 31: 2629-47. PMID: 22510884
- Novel mechanisms of resistance to vemurafenib in melanoma - V600E B-Raf reversion and switching VEGF-A splice isoform expression. | Beazley-Long, N., et al. 2015. Am J Cancer Res. 5: 433-41. PMID: 25628951
- Design and discovery of thioether and nicotinamide containing sorafenib analogues as multikinase inhibitors targeting B-Raf, B-RafV600E and VEGFR-2. | Sun, S., et al. 2018. Bioorg Med Chem. 26: 2381-2391. PMID: 29631788
- Structural optimization of imidazothiazole derivatives affords a new promising series as B-Raf V600E inhibitors; synthesis, in vitro assay and in silico screening. | Ammar, UM., et al. 2020. Bioorg Chem. 100: 103967. PMID: 32470760
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRAF Antibody (13) | sc-136263 | 50 µg/0.5 ml | $322.00 |