Rae-1β/γ Antibody (Xc-12): sc-80310

Rae-1β/γ Antibody (Xc-12) is a rat monoclonal IgG2a antibody that detects Rae-1 beta/gamma protein of mouse and rat origin by flow cytometry (FCM) and enzyme-linked immunosorbent assay (ELISA). Anti-Rae-1 beta/gamma antibody (Xc-12) is available as the non-conjugated form, providing researchers with a reliable tool for studying the interactions between natural killer (NK) cells and their ligands. Rae-1 beta/gamma protein plays a crucial role in immune surveillance, as a ligand for the NKG2D receptor, expressed on NK cells and certain T cells. Rae-1 beta/gamma expression on tumor cells can trigger an immune response, making this protein an important factor in tumor immunity. Rae-1 beta/gamma belongs to a family of ligands including H60, which compete for binding to NKG2D, with NKG2D showing significantly higher affinity for H60. This competition helps explain how NK cells recognize and eliminate tumor cells, as Rae-1 beta/gamma and H60 expression remains low in normal tissues but increases in response to stress or transformation, enhancing tumor cell susceptibility to NK cell-mediated lysis. Rae-1 beta/gamma′s ability to modulate immune responses highlights its potential as a target for therapeutic strategies aimed at enhancing anti-tumor immunity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rae-1β/γ Antibody (Xc-12) References:

1. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice.  |  Cerwenka, A., et al. 2000. Immunity. 12: 721-7. PMID: 10894171
2. Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages.  |  Diefenbach, A., et al. 2000. Nat Immunol. 1: 119-26. PMID: 11248803
3. Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family.  |  Steinle, A., et al. 2001. Immunogenetics. 53: 279-87. PMID: 11491531
4. Molecular competition for NKG2D: H60 and RAE1 compete unequally for NKG2D with dominance of H60.  |  O'Callaghan, CA., et al. 2001. Immunity. 15: 201-11. PMID: 11520456
5. Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity.  |  Diefenbach, A., et al. 2001. Nature. 413: 165-71. PMID: 11557981
6. Crystal structures of RAE-1beta and its complex with the activating immunoreceptor NKG2D.  |  Li, P., et al. 2002. Immunity. 16: 77-86. PMID: 11825567
7. Ligands for murine NKG2D display heterogeneous binding behavior.  |  Carayannopoulos, LN., et al. 2002. Eur J Immunol. 32: 597-605. PMID: 11857333
8. Natural killer cell-mediated lysis of dorsal root ganglia neurons via RAE1/NKG2D interactions.  |  Backström, E., et al. 2003. Eur J Immunol. 33: 92-100. PMID: 12594837
9. Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: a novel cDNA family encodes cell surface proteins sharing partial homology with MHC class I molecules.  |  Zou, Z., et al. 1996. J Biochem. 119: 319-28. PMID: 8882725