Rad51 Double Nickase Plasmid (h): sc-400100-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rad51 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rad51 Double Nickase Plasmid (h) and Rad51 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAD51. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rad51 Antibody (F-11): sc-398587
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad51 Double Nickase Plasmid (h) | sc-400100-NIC | 20 µg | $410.00 | |||
Rad51 Double Nickase Plasmid (h2) | sc-400100-NIC-2 | 20 µg | $410.00 |
Human RAD51 encodes Rad51, a central recombinase that catalyzes homology search and DNA strand exchange during homologous recombination, enabling high-fidelity repair of DNA double-strand breaks and stalled replication forks. Rad51 functions downstream of BRCA1/BRCA2 and coordinates with RAD52, PALB2, and replication-associated factors to preserve genome stability across S and G2 phases. Through its roles in replication stress responses, meiotic recombination, and chromatin-based DNA repair, RAD51 activity influences checkpoint signaling and sister chromatid exchange. Dysregulation of RAD51 expression or function is linked to genomic instability phenotypes and is frequently studied in the context of tumor biology, inherited DNA repair defects, and sensitivity to DNA-damaging agents in experimental systems.
Rad51 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAD51 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAD51. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAD51 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAD51-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.