Rac 1 Double Nickase Plasmid (h): sc-400175-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rac 1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rac 1 Double Nickase Plasmid (h) and Rac 1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rac 1/2/3 Antibody (G-2): sc-514583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rac 1 Double Nickase Plasmid (h) | sc-400175-NIC | 20 µg | $410.00 | |||
Rac 1 Double Nickase Plasmid (h2) | sc-400175-NIC-2 | 20 µg | $410.00 |
RAC1 encodes Rac1, a Rho family small GTPase that cycles between active GTP-bound and inactive GDP-bound states to coordinate actin cytoskeleton remodeling, membrane ruffling, and cell polarity. Rac1 signals through effectors such as PAK kinases, WAVE regulatory complex, and NADPH oxidase, integrating cues from receptor tyrosine kinases and integrins to regulate migration, adhesion, endocytosis, and reactive oxygen species generation. In human biology, dysregulated RAC1 activity is linked to altered epithelial–mesenchymal dynamics, invasion programs, and immune cell trafficking, and has been associated with cancer progression, neurodevelopmental disorders, and inflammatory phenotypes. These pathway connections make RAC1 a widely used node for interrogating cytoskeletal control, MAPK crosstalk, and cell-state transitions in mechanistic studies.
Rac 1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.