rabphilin-3A Antibody (D-6): sc-393197
- rabphilin-3A Antibody (D-6) is a mouse monoclonal IgG2a κ rabphilin-3A antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 516-576 mapping within an internal region of rabphilin-3A of human origin
- rabphilin-3A Antibody (D-6) is recommended for detection of rabphilin-3A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-rabphilin-3A Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for rabphilin-3A Antibody (D-6). This work is in progress.
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rabphilin-3A Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects rabphilin-3A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-rabphilin-3A antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. rabphilin-3A plays a crucial role in calcium-dependent exocytosis, acting as a positive regulator in this process. rabphilin-3A is primarily located in neuroendocrine cells, where rabphilin-3A co-localizes with Rab3A on synaptic vesicles and chromaffin granules, highlighting rabphilin-3A′s importance in neurotransmitter release and hormone secretion. rabphilin-3A′s structure includes two C2 domains at the carboxy-terminal and a cysteine-rich region with two zinc finger motifs at the amino-terminal, which are essential for interaction with calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles. These structural features enable rabphilin-3A to bind to Rab3A in a GTP/Mg²⁺-dependent manner, facilitating the regulation of exocytosis. While rabphilin-3A and Rab3A interact, both appear to influence exocytosis independently, suggesting that rabphilin-3A may mediate effects through interactions with an unidentified factor that recognizes the Rab3 binding domain. rabphilin-3A monoclonal antibody (D-6) serves as an invaluable tool for researchers studying the mechanisms of exocytosis and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
rabphilin-3A Antibody (D-6) References:
- Comparison of the effects on secretion in chromaffin and PC12 cells of Rab3 family members and mutants. Evidence that inhibitory effects are independent of direct interaction with Rabphilin3. | Chung, SH., et al. 1999. J Biol Chem. 274: 18113-20. PMID: 10364266
- High affinity Rab3 binding is dispensable for Rabphilin-dependent potentiation of stimulated secretion. | Joberty, G., et al. 1999. J Cell Sci. 112 (Pt 20): 3579-87. PMID: 10504306
- Structure of the Janus-faced C2B domain of rabphilin. | Ubach, J., et al. 1999. Nat Cell Biol. 1: 106-12. PMID: 10559882
- Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins. | Fukuda, M. and Mikoshiba, K. 2001. Biochem Biophys Res Commun. 281: 1226-33. PMID: 11243866
- The C2 domains of Rabphilin3A specifically bind phosphatidylinositol 4,5-bisphosphate containing vesicles in a Ca2+-dependent manner. In vitro characteristics and possible significance. | Chung, SH., et al. 1998. J Biol Chem. 273: 10240-8. PMID: 9553075
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rabphilin-3A Antibody (D-6) | sc-393197 | 200 µg/ml | $322.00 | |||
rabphilin-3A Antibody (D-6) AC | sc-393197 AC | 500 µg/ml, 25% agarose | $424.00 | |||
rabphilin-3A Antibody (D-6) HRP | sc-393197 HRP | 200 µg/ml | $322.00 | |||
rabphilin-3A Antibody (D-6) FITC | sc-393197 FITC | 200 µg/ml | $336.00 | |||
rabphilin-3A Antibody (D-6) PE | sc-393197 PE | 200 µg/ml | $349.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 488 | sc-393197 AF488 | 200 µg/ml | $364.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 546 | sc-393197 AF546 | 200 µg/ml | $364.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 594 | sc-393197 AF594 | 200 µg/ml | $364.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 647 | sc-393197 AF647 | 200 µg/ml | $364.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 680 | sc-393197 AF680 | 200 µg/ml | $364.00 | |||
rabphilin-3A Antibody (D-6) Alexa Fluor® 790 | sc-393197 AF790 | 200 µg/ml | $364.00 |