Date published: 2025-10-19

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Rab 7b Antibody (JK-17): sc-81922

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Datasheets
  • Rab 7b Antibody (JK-17) is a mouse monoclonal IgG2a κ Rab 7b antibody, cited in 4 publications, provided at 100 µg/ml
  • raised against recombinant Rab 7b of human origin
  • recommended for detection of Rab 7b of human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rab 7b Antibody (JK-17). This work is in progress.

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Rab 7b Antibody (JK-17) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rab 7b protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 7b antibody (JK-17) is available as a non-conjugated format. Rab 7b, a member of the Ras-related superfamily of guanine nucleotide-binding proteins, plays a crucial role in intracellular transport processes, particularly in the endocytic pathway. Rab 7b is essential for transporting newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus and ultimately to secretory vesicles, a process relying on carrier vesicle movement facilitated by Rab proteins. Rab 7b shares significant homology with Rab 7, another Rab GTPase, underscoring Rab 7b′s importance in regulating vesicular traffic. Rab 7b is predominantly expressed in tissues such as lung, placenta, skeletal muscle, heart, and peripheral blood leukocytes, where Rab 7b influences monocyte functions, including endocytosis and protein antigen presentation. Rab 7b undergoes post-translational modifications, including phosphorylation by protein kinase C and casein kinase II, which are critical for Rab 7b′s function as a Rab GTPase. Rab 7b′s interactions with various proteins involved in these pathways highlight Rab 7b′s significance in cellular transport mechanisms and immune responses.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Rab 7b Antibody (JK-17) References:

  1. Cloning and characterization of a gene (avaA) from Aspergillus nidulans encoding a small GTPase involved in vacuolar biogenesis.  |  Ohsumi, K., et al. 2002. Gene. 291: 77-84. PMID: 12095681
  2. Rab7b, a novel lysosome-associated small GTPase, is involved in monocytic differentiation of human acute promyelocytic leukemia cells.  |  Yang, M., et al. 2004. Biochem Biophys Res Commun. 318: 792-9. PMID: 15144907
  3. Cloning and characterization of a novel splice variant of human Rab18 gene (RAB18).  |  Dou, T., et al. 2005. DNA Seq. 16: 230-4. PMID: 16147880
  4. Cloning of two genes encoding Rab7 in Paramecium.  |  Surmacz, L., et al. 2006. Acta Biochim Pol. 53: 149-56. PMID: 16365637
  5. The DNA sequence and biological annotation of human chromosome 1.  |  Gregory, SG., et al. 2006. Nature. 441: 315-21. PMID: 16710414
  6. Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4.  |  Wang, Y., et al. 2007. Blood. 110: 962-71. PMID: 17395780
  7. Localization of MAP1-LC3 in vulnerable neurons and Lewy bodies in brains of patients with dementia with Lewy bodies.  |  Higashi, S., et al. 2011. J Neuropathol Exp Neurol. 70: 264-80. PMID: 21412173
  8. Impact of clopidogrel and aspirin treatment on the expression of proteins in platelets from type-2 diabetic patients with stable coronary ischemia.  |  Azcona, L., et al. 2012. J Pharm Sci. 101: 2821-32. PMID: 22614874
  9. Acute coronary syndrome remodels the protein cargo and functions of high-density lipoprotein subfractions.  |  Tan, Y., et al. 2014. PLoS One. 9: e94264. PMID: 24736723
  10. Identification of potential biomarkers and metabolic pathways based on integration of metabolomic and transcriptomic data in the development of breast cancer.  |  Yang, Y., et al. 2021. Arch Gynecol Obstet. 303: 1599-1606. PMID: 33791842
  11. Analysis of Gtpases Rab 5 and Rab 7 expression from macrophages infected with biofilm-producing and non biofilm-producing strains of Corynebacterium pseudotuberculosis.  |  da Conceição Aquino de Sá, M., et al. 2022. Braz J Microbiol. 53: 447-453. PMID: 35023082
  12. The HEAT repeat protein HPO-27 is a lysosome fission factor.  |  Li, L., et al. 2024. Nature. 628: 630-638. PMID: 38538795

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rab 7b Antibody (JK-17)

sc-81922
100 µg/ml
$333.00

Have you confirmed that this antibody(sc-81922) is specific for Rab7b and does not cross-react with Rab7a? Any hint of where the epitope is for this Ab?

Asked by: Ralph
Thank you for your question. The epitope range is 100-200. Here is a link for more information on the protein : https://www.uniprot.org/uniprotkb/Q96AH8/entry.
Answered by: BlakeJ
Date published: 2024-08-12

Can sc-81922: Rab 7b (JK-17) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, sc-81922: Rab 7b (JK-17) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for immunofluorescenceThis antibody has been used for immunofluorescence in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from western blotHEK細胞を用いて通常のウエスタンブロットを行い、予想される高さにクリアなシングルバンドを認めました。
Date published: 2018-10-24
Rated 5 out of 5 by from Worked for IF with paraformaldehydeWorked for IF with paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Very nice looking Western using HeLa wholeVery nice looking Western using HeLa whole cell lysate. -SCBT QC
Date published: 2014-09-20
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Rab 7b Antibody (JK-17) is rated 5.0 out of 5 by 5.
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