Rab 44 Lentiviral Activation Particles (h): sc-418677-LAC

RAB44 encodes Rab 44, an atypical Rab family small GTPase implicated in regulation of intracellular membrane trafficking, vesicle maturation, and endolysosomal dynamics. As a switch protein cycling between GTP- and GDP-bound states, Rab 44 is thought to influence compartment identity and cargo routing that intersect with secretory and degradative pathways, including processes coupled to immune cell function and inflammatory signaling. Dysregulated Rab GTPase activity can perturb receptor turnover, antigen processing, and signal transduction, making RAB44 a relevant target for studies of pathway rewiring in cancer and immune-associated disease contexts. Functional interrogation of RAB44 supports mechanistic analyses linking trafficking control to cellular activation states, stress responses, and proteostasis.

Rab 44 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient RAB44 upregulation across a broader range of human cell types.

Rab 44 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the RAB44 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Rab 44 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native RAB44 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.