Rab 3B Antibody (48-K2): sc-81911

Rab 3B Antibody (48-K2) is a mouse monoclonal IgG2a antibody that detects Rab 3B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Rab 3B antibody (48-K2) is available as a non-conjugated format. Rab 3B plays a crucial role in regulating vesicle trafficking, particularly in neurotransmitter and hormone transport, which is essential for proper cellular communication and function. Located primarily in the cytoplasm and associated with secretory vesicles, Rab 3B is involved in docking and fusion of vesicles with target membranes, facilitating content release into extracellular space. This process is vital for neurotransmission and hormone secretion, highlighting Rab 3B′s importance in maintaining physiological homeostasis. Rab 3B belongs to the Ras-related superfamily of guanine nucleotide-binding proteins, exhibiting significant homology with Ras p21, and functions with various interacting proteins that assist in vesicle transport. With at least eight members identified in the Rab family, each protein localizes to specific stages of membrane transport pathways, underscoring the complexity and specificity of intracellular trafficking mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rab 3B Antibody (48-K2) References:

1. GTP-binding proteins in intracellular transport.  |  Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
2. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach.  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
3. Cloning of a Rab3 isotype predominantly expressed in adipocytes.  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
4. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
5. Encoding gene RAB3B exists in linear chromosomal and circular extrachromosomal DNA and contributes to cisplatin resistance of hypopharyngeal squamous cell carcinoma via inducing autophagy.  |  Lin, C., et al. 2022. Cell Death Dis. 13: 171. PMID: 35194030
6. Rab3B enhances the stabilization of DDX6 to promote lung adenocarcinoma aggressiveness.  |  Yao, G., et al. 2024. Mol Med. 30: 75. PMID: 38834947
7. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
8. Coatomers and SNAREs in promoting membrane traffic.  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
9. Friends and family: the role of the Rab GTPases in vesicular traffic.  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
10. The role of GTP-binding proteins in transport along the exocytic pathway.  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
11. Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation.  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
12. Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins.  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

The preferred Rab 3/16 antibody is Rab 3/16 Antibody (G-1): sc-271044.