Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rab 11A Double Nickase Plasmid (h): sc-400617-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rab 11A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rab 11A Double Nickase Plasmid (h) and Rab 11A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RAB11A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rab 11A Antibody (D-3): sc-166523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rab 11A Double Nickase Plasmid (h) | sc-400617-NIC | 20 µg | $410.00 | |||
Rab 11A Double Nickase Plasmid (h2) | sc-400617-NIC-2 | 20 µg | $410.00 |
RAB11A encodes the small GTPase Rab 11A, a central regulator of recycling endosome dynamics and membrane trafficking that coordinates endocytic recycling, vesicle budding, and cargo delivery to the plasma membrane. Rab 11A functions with Rab11 family–interacting proteins and the exocyst to control receptor recycling, integrin turnover, polarized transport, and cytokinetic abscission, linking it to cytoskeletal remodeling and cell migration. Through its roles in sorting and trafficking of signaling receptors and junctional components, Rab 11A influences pathways that shape epithelial polarity and growth factor responsiveness. Dysregulated Rab11A-dependent trafficking has been associated with altered invasion and metastatic behavior in cancer models, and perturbed endosomal recycling has also been implicated in neurodegenerative and infectious disease mechanisms.
Rab 11A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RAB11A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RAB11A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RAB11A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RAB11A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.