QIP1 Antibody (3D10): sc-101547

QIP1 Antibody (3D10) is a rat monoclonal IgG2a antibody that detects QIP1 protein of mouse, rat, human, hamster, bovine, and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). QIP1 (3D10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. QIP1, also known as KPNA4 (karyopherin α4), IPOA3 (importin α3), or SRP3, plays a crucial role in nuclear import by forming a complex with importin β proteins, specifically karyopherin β1, which acts as an adapter. This interaction is vital for the transport of proteins containing nuclear localization signal (NLS) motifs into the nucleus, as karyopherin β1 facilitates the binding of the importin/substrate complex to the nuclear pore complex (NPC). The translocation of this complex through the pore is mediated by a Ran-dependent mechanism, ensuring that essential proteins are accurately delivered to the nucleus for proper cellular function. QIP1 features an IBB domain at its hydrophilic N-terminus, which is essential for binding to karyopherin β1, along with ten ARM repeats in its hydrophobic central region that contribute to structural integrity. QIP1 is highly expressed in various tissues, including the pancreas, lung, ovary, testis, small intestine, heart, and skeletal muscle, where QIP1 exhibits both cytoplasmic and nuclear localization, underscoring importance in cellular transport processes.

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QIP1 Antibody (3D10) References:

1. Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import.  |  Köhler, M., et al. 1999. Mol Cell Biol. 19: 7782-91. PMID: 10523667
2. Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, by in situ hybridization.  |  Ayala-Madrigal, ML., et al. 2000. Cytogenet Cell Genet. 89: 258-9. PMID: 10965137
3. Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.  |  Sakaguchi, N., et al. 2003. Hybrid Hybridomics. 22: 397-400. PMID: 14683601
4. The nuclear import of RNA helicase A is mediated by importin-alpha3.  |  Aratani, S., et al. 2006. Biochem Biophys Res Commun. 340: 125-33. PMID: 16375861
5. Differential regulation of karyopherin alpha 2 expression by TGF-beta1 and IFN-gamma in normal human epidermal keratinocytes: evident contribution of KPNA2 for nuclear translocation of IRF-1.  |  Umegaki, N., et al. 2007. J Invest Dermatol. 127: 1456-64. PMID: 17255955
6. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.  |  Seki, T., et al. 1997. Biochem Biophys Res Commun. 234: 48-53. PMID: 9168958
7. Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors.  |  Miyamoto, Y., et al. 1997. J Biol Chem. 272: 26375-81. PMID: 9334211
8. Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.  |  Köhler, M., et al. 1997. FEBS Lett. 417: 104-8. PMID: 9395085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-QIP1 Antibody (3D10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-QIP1 Antibody (3D10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

QIP1 Antibody (3D10) Specifications

Alternate Product Name: QIP1

Product Clone Name: 3D10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: QIP1

Epitope Species: mouse

Product Reactivity/Detection: QIP1

Species Reactivity/Detection: mouse, rat, human, hamster, bovine, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for QIP1 Antibody (3D10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin