pyridoxal kinase Antibody (E-2): sc-365173
- pyridoxal kinase Antibody (E-2) is a mouse monoclonal IgG2b κ pyridoxal kinase antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 221-312 mapping at the C-terminus of pyridoxal kinase of human origin
- pyridoxal kinase Antibody (E-2) is recommended for detection of pyridoxal kinase of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-pyridoxal kinase Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for pyridoxal kinase Antibody (E-2) for WB applications. This reagent is now offered in a bundle with pyridoxal kinase Antibody (E-2) (see ordering information below).
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pyridoxal kinase Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects pyridoxal kinase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). pyridoxal kinase (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pyridoxal kinase plays a crucial role in the phosphorylation of vitamin B6, which is essential for its conversion to pyridoxal 5′ phosphate (PLP), the biologically active form of vitamin B6. PLP serves as a coenzyme in numerous enzymatic reactions, including those involved in amino acid metabolism, neurotransmitter synthesis, and the production of hemoglobin, thereby maintaining overall metabolic homeostasis. Pyridoxal kinase consists of 312 amino acids and is predominantly located in the cytoplasm, where pyridoxal kinase may function as a homodimer. Notably, three isoforms of pyridoxal kinase exist, with isoform 3 being specifically expressed in adult testis and spermatozoa, highlighting pyridoxal kinase′s potential role in reproductive biology. PDXK, located on chromosome 21q22.3, has been implicated as a candidate gene for autoimmune polyglandular disease type 1, a genetic disorder associated with this chromosomal region. Pyridoxal kinase exhibits optimal activity at a pH range of 5.5 to 6.0, making pyridoxal kinase particularly relevant in physiological conditions where pH may fluctuate.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
pyridoxal kinase Antibody (E-2) References:
- Human pyridoxal kinase: overexpression and properties of the recombinant enzyme. | Lee, HS., et al. 2000. Mol Cells. 10: 452-9. PMID: 10987144
- Expression of a novel pyridoxal kinase mRNA splice variant, PKH-T, in human testis. | Fang, X., et al. 2004. Asian J Androl. 6: 83-91. PMID: 15154080
- Roscovitine targets, protein kinases and pyridoxal kinase. | Bach, S., et al. 2005. J Biol Chem. 280: 31208-19. PMID: 15975926
- Crystal structure of pyridoxal kinase in complex with roscovitine and derivatives. | Tang, L., et al. 2005. J Biol Chem. 280: 31220-9. PMID: 15985434
- Age-dependent changes of pyridoxal phosphate synthesizing enzymes immunoreactivities and activities in the gerbil hippocampal CA1 region. | Hwang, IK., et al. 2005. Mech Ageing Dev. 126: 1322-30. PMID: 16207494
- A novel role of circadian transcription factor DBP in hippocampal plasticity. | Klugmann, M., et al. 2006. Mol Cell Neurosci. 31: 303-14. PMID: 16257226
- Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor. | Hanna, MC., et al. 1997. J Biol Chem. 272: 10756-60. PMID: 9099727
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pyridoxal kinase Antibody (E-2) | sc-365173 | 200 µg/ml | $322.00 | |||
pyridoxal kinase Antibody (E-2): m-IgG2b BP-HRP Bundle | sc-548720 | 200 µg Ab; 10 µg BP | $361.00 | |||
pyridoxal kinase Antibody (E-2) AC | sc-365173 AC | 500 µg/ml, 25% agarose | $424.00 | |||
pyridoxal kinase Antibody (E-2) HRP | sc-365173 HRP | 200 µg/ml | $322.00 | |||
pyridoxal kinase Antibody (E-2) FITC | sc-365173 FITC | 200 µg/ml | $336.00 | |||
pyridoxal kinase Antibody (E-2) PE | sc-365173 PE | 200 µg/ml | $349.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 488 | sc-365173 AF488 | 200 µg/ml | $364.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 546 | sc-365173 AF546 | 200 µg/ml | $364.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 594 | sc-365173 AF594 | 200 µg/ml | $364.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 647 | sc-365173 AF647 | 200 µg/ml | $364.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 680 | sc-365173 AF680 | 200 µg/ml | $364.00 | |||
pyridoxal kinase Antibody (E-2) Alexa Fluor® 790 | sc-365173 AF790 | 200 µg/ml | $364.00 |