Pygopus 1 CRISPR/Cas9 KO Plasmid (h): sc-405936
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pygopus 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pygopus 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pygopus 1 Antibody (3E1): sc-517079
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pygopus 1 CRISPR/Cas9 KO Plasmid (h) | sc-405936 | 20 µg | $397.00 |
Overview
PYGO1 encodes Pygopus 1, a nuclear co-activator that couples β-catenin/TCF transcriptional complexes to chromatin in canonical Wnt signaling. Through interactions with BCL9/9L and histone-modifying machinery, Pygopus 1 supports transcriptional programs controlling cell fate specification, proliferation, and epithelial homeostasis. PYGO1-dependent Wnt outputs intersect with processes such as stem-like maintenance and differentiation, and altered regulation of this pathway is frequently implicated in oncogenic transcriptional states and developmental dysregulation. These features make PYGO1 a useful target for dissecting Wnt-driven gene expression and chromatin engagement in human cellular models.
Pygopus 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PYGO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PYGO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PYGO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pygopus 1 protein expression.
This CRISPR knockout system enables efficient generation of PYGO1-deficient cell models for investigation of Pygopus 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PYGO1 exon(s) critical for Pygopus 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PYGO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pygopus 1 CRISPR/Cas9 KO Plasmid (h) and Pygopus 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PYGO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pygopus 1 HDR Plasmid (h) and Pygopus 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PYGO1 homology arms to support homology-directed repair at defined PYGO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.