Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PXR Double Nickase Plasmid (h): sc-400824-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PXR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PXR Double Nickase Plasmid (h) and PXR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR1I2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PXR Antibody (G-11): sc-48403
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PXR Double Nickase Plasmid (h) | sc-400824-NIC | 20 µg | $410.00 | |||
PXR Double Nickase Plasmid (h2) | sc-400824-NIC-2 | 20 µg | $410.00 |
NR1I2 encodes pregnane X receptor (PXR), a ligand-activated nuclear receptor that functions as a xenobiotic sensor linking chemical exposure to transcriptional control of detoxification programs. Upon activation, PXR heterodimerizes with RXR and binds response elements to regulate genes involved in phase I/II metabolism and transport, including CYP3A, UGTs, and ABC transporters, integrating with bile acid, lipid, and inflammatory signaling pathways. This regulatory network influences hepatic and intestinal homeostasis and shapes cellular responses to drugs and environmental chemicals. Dysregulated PXR signaling has been associated with variability in drug metabolism and interactions, cholestatic and metabolic phenotypes, and context-dependent effects in inflammation-associated conditions and cancer biology.
PXR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR1I2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR1I2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR1I2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR1I2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.