Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.
1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187
See how others have used Puromycin dihydrochloride (CAS 58-58-2). Click on the entry to view the PubMed entry .
PMID: # 35586315 Elías-Villalobos, A.|Duncan, C.|Mata, J.|Helmlinger, D.| et al. 2022. STAR Protoc. 3: 101373.
PMID: # 35263578 Michl, J.|Wang, Y.|Monterisi, S.|Blaszczak, W.|Beveridge, R.|Bridges, EM.|Koth, J.|Bodmer, WF.|Swietach, P.| et al. 2022. Cell Rep. 38: 110493.
PMID: # 35216678 Liao, P.|Wang, W.|Wang, W.|Kryczek, I.|Li, X.|Bian, Y.|Sell, A.|Wei, S.|Grove, S.|Johnson, JK.|Kennedy, PD.|Gijón, M.|Shah, YM.|Zou, W.| et al. 2022. Cancer Cell.
PMID: # 35163006 Papasavva, PL.|Patsali, P.|Loucari, CC.|Kurita, R.|Nakamura, Y.|Kleanthous, M.|Lederer, CW.| et al. 2022. Int J Mol Sci. 23:
PMID: # 35104602 Betlej, G.|Lewińska, A.|Adamczyk-Grochala, J.|Błoniarz, D.|Rzeszutek, I.|Wnuk, M.| et al. 2022. Toxicol In Vitro. 80: 105323.
PMID: # 35053476 Zerfaoui, M.|Toraih, E.|Ruiz, E.|Errami, Y.|Attia, AS.|Krzysztof, M.|Abd Elmageed, ZY.|Kandil, E.| et al. 2022. Cancers (Basel). 14:
PMID: # 35581596 Suárez-Rivero, JM.|Pastor-Maldonado, CJ.|Povea-Cabello, S.|Álvarez-Córdoba, M.|Villalón-García, I.|Talaverón-Rey, M.|Suárez-Carrillo, A.|Munuera-Cabeza, M.|Reche-López, D.|Cilleros-Holgado, P.|Piñero-Perez, R.|Sánchez-Alcázar, JA.| et al. 2022. Orphanet J Rare Dis. 17: 204.
PMID: # 35045282 Chattopadhyay, M.|Jenkins, EC.|Lechuga-Vieco, AV.|Nie, K.|Fiel, MI.|Rialdi, A.|Guccione, E.|Enriquez, JA.|Sia, D.|Lujambio, A.|Germain, D.| et al. 2022. Cell Rep. 38: 110254.
PMID: # 35433850 Granata, A.|Kasioulis, I.|Serrano, F.|Cooper, JD.|Traylor, M.|Sinha, S.|Markus, HS.| et al. 2022. Front Cardiovasc Med. 9: 849664.
PMID: # 34192330 Oliveira, RM.|Teixeira, TL.|Rodrigues, CC.|da Silva, AA.|Borges, BC.|Brígido, RTS.|Teixeira, SC.|Dos Santos, MA.|Servato, JPS.|de O Santos, D.|Silva, MJB.|Goulart, LR.|da Silva, CV.| et al. 2021. Glycobiology. 31: 1378-1389.