Date published: 2026-4-24

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PU.1/Spi1 Antibody (B-9): sc-390659

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Datasheets
  • PU.1/Spi1 Antibody (B-9) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 239-271 at the C-terminus of PU.1 of mouse origin
  • recommended for detection of PU.1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • TransCruz reagent for ChIP application (sc-390659 X, 200 µg/0.1 ml)
  • See PU.1/Spi1 (C-3): sc-390405 for PU.1/Spi1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PU.1/Spi1 Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with PU.1/Spi1 Antibody (B-9) (see ordering information below).
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PU.1 Antibody (B-9) is a mouse monoclonal IgG1 antibody that detects PU.1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PU.1 is a critical transcription factor belonging to the Ets family, playing a vital role in the regulation of gene expression during lymphoid development and immune response. PU.1 is primarily located in the nucleus, where PU.1 binds to specific DNA sequences to activate or repress target genes essential for B cell differentiation and function. PU.1′s nuclear localization enables interaction with other transcription factors and co-regulators, facilitating the transcriptional programs necessary for hematopoiesis and immune cell maturation. PU.1 is expressed in a diverse range of hematopoietic cells, including B cells, early T-cells, and myeloid cells, underscoring PU.1′s pivotal role in the immune system. Anti-PU.1 antibody (B-9) serves as an invaluable tool for researchers studying the mechanisms of immune cell development and function, as well as the underlying pathways involved in various hematological disorders.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PU.1/Spi1 Antibody (B-9)

sc-390659
200 µg/ml
$322.00

PU.1/Spi1 Antibody (B-9): m-IgG Fc BP-HRP Bundle

sc-540830
200 µg Ab; 10 µg BP
$361.00

PU.1/Spi1 Antibody (B-9): m-IgG1 BP-HRP Bundle

sc-542399
200 µg Ab; 20 µg BP
$361.00

PU.1/Spi1 (B-9) Neutralizing Peptide

sc-390659 P
100 µg/0.5 ml
$69.00

PU.1/Spi1 Antibody (B-9) X

sc-390659 X
200 µg/0.1 ml
$322.00

What application is the blocking peptide sc-390659 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great monoclonal antibodyNice strong signal by Western blot with little to no background. Strong robust signal in IHCP
Date published: 2017-02-06
Rated 5 out of 5 by from Satisfactory Western blot data of PUSatisfactory Western blot data of PU.1 expression in K-562 nuclear extract. -SCBT QC
Date published: 2013-05-07
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PU.1/Spi1 Antibody (B-9) is rated 5.0 out of 5 by 2.
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